Transcriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.
|Original language||English (US)|
|Number of pages||9|
|Journal||Molecular and Cellular Biology|
|State||Published - Dec 15 2014|
Bibliographical noteKAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was partially supported by grants from ERC (ERC-2013-AdG340172-MUNCODD), Telethon (GGP11149), the Epigen-Epigenomics Flagship Project, Parent Project Italia, and PRIN to I.B. and KAUST and PRIN to A.T.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.