Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development

Bao Jun Yang, Max Minne, Federica Brunoni, Lenka Plačková, Ivan Petřík, Yanbiao Sun, Jonah Nolf, Wouter Smet, Kevin Verstaen, Jos R. Wendrich, Thomas Eekhout, Klára Hoyerová, Gert Van Isterdael, Jurgen Haustraete, Anthony Bishopp, Etienne Farcot, Ondřej Novák, Yvan Saeys, Bert De Rybel

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

During plant development, a precise balance of cytokinin is crucial for correct growth and patterning, but it remains unclear how this is achieved across different cell types and in the context of a growing organ. Here we show that in the root apical meristem, the TMO5/LHW complex increases active cytokinin levels via two cooperatively acting enzymes. By profiling the transcriptomic changes of increased cytokinin at single-cell level, we further show that this effect is counteracted by a tissue-specific increase in CYTOKININ OXIDASE 3 expression via direct activation of the mobile transcription factor SHORTROOT. In summary, we show that within the root meristem, xylem cells act as a local organizer of vascular development by non-autonomously regulating cytokinin levels in neighbouring procambium cells via sequential induction and repression modules.
Original languageEnglish (US)
Pages (from-to)1485-1494
Number of pages10
JournalNature Plants
Volume7
Issue number11
DOIs
StatePublished - Nov 1 2021

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-10-23

Fingerprint

Dive into the research topics of 'Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development'. Together they form a unique fingerprint.

Cite this