Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development

Bao Jun Yang; Max Minne; Federica Brunoni; Lenka Plačková; Ivan Petřík; Yanbiao Sun; Jonah Nolf; Wouter Smet; Kevin Verstaen; Jos R. Wendrich; Thomas Eekhout; Klára Hoyerová; Gert Van Isterdael; Jurgen Haustraete; Anthony Bishopp; Etienne Farcot; Ondřej Novák; Yvan Saeys; Bert De Rybel

doi:10.1038/s41477-021-01017-6

Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development

Bao Jun Yang, Max Minne, Federica Brunoni, Lenka Plačková, Ivan Petřík, Yanbiao Sun, Jonah Nolf, Wouter Smet, Kevin Verstaen, Jos R. Wendrich, Thomas Eekhout, Klára Hoyerová, Gert Van Isterdael, Jurgen Haustraete, Anthony Bishopp, Etienne Farcot, Ondřej Novák, Yvan Saeys, Bert De Rybel

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40 Scopus citations

Abstract

During plant development, a precise balance of cytokinin is crucial for correct growth and patterning, but it remains unclear how this is achieved across different cell types and in the context of a growing organ. Here we show that in the root apical meristem, the TMO5/LHW complex increases active cytokinin levels via two cooperatively acting enzymes. By profiling the transcriptomic changes of increased cytokinin at single-cell level, we further show that this effect is counteracted by a tissue-specific increase in CYTOKININ OXIDASE 3 expression via direct activation of the mobile transcription factor SHORTROOT. In summary, we show that within the root meristem, xylem cells act as a local organizer of vascular development by non-autonomously regulating cytokinin levels in neighbouring procambium cells via sequential induction and repression modules.

Original language	English (US)
Pages (from-to)	1485-1494
Number of pages	10
Journal	Nature Plants
Volume	7
Issue number	11
DOIs	https://doi.org/10.1038/s41477-021-01017-6
State	Published - Nov 1 2021

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Yang, B. J., Minne, M., Brunoni, F., Plačková, L., Petřík, I., Sun, Y., Nolf, J., Smet, W., Verstaen, K., Wendrich, J. R., Eekhout, T., Hoyerová, K., Van Isterdael, G., Haustraete, J., Bishopp, A., Farcot, E., Novák, O., Saeys, Y., & De Rybel, B. (2021). Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development. Nature Plants, 7(11), 1485-1494. https://doi.org/10.1038/s41477-021-01017-6

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title = "Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development",

abstract = "During plant development, a precise balance of cytokinin is crucial for correct growth and patterning, but it remains unclear how this is achieved across different cell types and in the context of a growing organ. Here we show that in the root apical meristem, the TMO5/LHW complex increases active cytokinin levels via two cooperatively acting enzymes. By profiling the transcriptomic changes of increased cytokinin at single-cell level, we further show that this effect is counteracted by a tissue-specific increase in CYTOKININ OXIDASE 3 expression via direct activation of the mobile transcription factor SHORTROOT. In summary, we show that within the root meristem, xylem cells act as a local organizer of vascular development by non-autonomously regulating cytokinin levels in neighbouring procambium cells via sequential induction and repression modules.",

author = "Yang, {Bao Jun} and Max Minne and Federica Brunoni and Lenka Pla{\v c}kov{\'a} and Ivan Pet{\v r}{\'i}k and Yanbiao Sun and Jonah Nolf and Wouter Smet and Kevin Verstaen and Wendrich, {Jos R.} and Thomas Eekhout and Kl{\'a}ra Hoyerov{\'a} and {Van Isterdael}, Gert and Jurgen Haustraete and Anthony Bishopp and Etienne Farcot and Ond{\v r}ej Nov{\'a}k and Yvan Saeys and {De Rybel}, Bert",

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day = "1",

doi = "10.1038/s41477-021-01017-6",

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Yang, BJ, Minne, M, Brunoni, F, Plačková, L, Petřík, I, Sun, Y, Nolf, J, Smet, W, Verstaen, K, Wendrich, JR, Eekhout, T, Hoyerová, K, Van Isterdael, G, Haustraete, J, Bishopp, A, Farcot, E, Novák, O, Saeys, Y & De Rybel, B 2021, 'Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development', Nature Plants, vol. 7, no. 11, pp. 1485-1494. https://doi.org/10.1038/s41477-021-01017-6

TY - JOUR

T1 - Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development

AU - Yang, Bao Jun

AU - Minne, Max

AU - Brunoni, Federica

AU - Plačková, Lenka

AU - Petřík, Ivan

AU - Sun, Yanbiao

AU - Nolf, Jonah

AU - Smet, Wouter

AU - Verstaen, Kevin

AU - Wendrich, Jos R.

AU - Eekhout, Thomas

AU - Hoyerová, Klára

AU - Van Isterdael, Gert

AU - Haustraete, Jurgen

AU - Bishopp, Anthony

AU - Farcot, Etienne

AU - Novák, Ondřej

AU - Saeys, Yvan

AU - De Rybel, Bert

N1 - Generated from Scopus record by KAUST IRTS on 2023-10-23

PY - 2021/11/1

Y1 - 2021/11/1

N2 - During plant development, a precise balance of cytokinin is crucial for correct growth and patterning, but it remains unclear how this is achieved across different cell types and in the context of a growing organ. Here we show that in the root apical meristem, the TMO5/LHW complex increases active cytokinin levels via two cooperatively acting enzymes. By profiling the transcriptomic changes of increased cytokinin at single-cell level, we further show that this effect is counteracted by a tissue-specific increase in CYTOKININ OXIDASE 3 expression via direct activation of the mobile transcription factor SHORTROOT. In summary, we show that within the root meristem, xylem cells act as a local organizer of vascular development by non-autonomously regulating cytokinin levels in neighbouring procambium cells via sequential induction and repression modules.

AB - During plant development, a precise balance of cytokinin is crucial for correct growth and patterning, but it remains unclear how this is achieved across different cell types and in the context of a growing organ. Here we show that in the root apical meristem, the TMO5/LHW complex increases active cytokinin levels via two cooperatively acting enzymes. By profiling the transcriptomic changes of increased cytokinin at single-cell level, we further show that this effect is counteracted by a tissue-specific increase in CYTOKININ OXIDASE 3 expression via direct activation of the mobile transcription factor SHORTROOT. In summary, we show that within the root meristem, xylem cells act as a local organizer of vascular development by non-autonomously regulating cytokinin levels in neighbouring procambium cells via sequential induction and repression modules.

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DO - 10.1038/s41477-021-01017-6

M3 - Article

SN - 2055-0278

VL - 7

SP - 1485

EP - 1494

JO - Nature Plants

JF - Nature Plants

IS - 11

ER -

Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development

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