Nitric oxide inhibits larval settlement in Amphibalanus amphitrite cyprids by repressing muscle locomotion and molting

Gen Zhang; Yue-Him Wong; Yu Zhang; Li-sheng He; Ying Xu; Pei-Yuan Qian

doi:10.1002/pmic.201500112

Nitric oxide inhibits larval settlement in Amphibalanus amphitrite cyprids by repressing muscle locomotion and molting

Gen Zhang, Yue-Him Wong, Yu Zhang, Li-sheng He, Ying Xu, Pei-Yuan Qian

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/NO (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecular mechanism, we analyzed changes in the proteome of A. amphitrite cyprids in response to different concentrations of the NO donor sodium nitroprusside (SNP; 62.5, 250 and 1000 μM) using a label-free proteomics method. Compared with the control, the expression of 106 proteins differed in all three treatments. These differentially expressed proteins were assigned to 13 pathways based on KEGG pathway enrichment analysis. SNP treatment stimulated the expression of heat shock proteins and arginine kinase, which are functionally related to NO synthases, increased the expression levels of glutathione transferases for detoxification, and activated the iron-mediated fatty acid degradation pathway and the citrate cycle through ferritin. Moreover, NO repressed the level of myosins and cuticular proteins, which indicated that NO might inhibit larval settlement in A. amphitrite by modulating the process of muscle locomotion and molting.

Original language	English (US)
Pages (from-to)	3854-3864
Number of pages	11
Journal	PROTEOMICS
Volume	15
Issue number	22
DOIs	https://doi.org/10.1002/pmic.201500112
State	Published - Oct 2 2015
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by grants from the China Ocean Mineral Resources Research and Development Association (DY125-15-T-02) and the Research Grants Council of the Hong Kong Special Administrative Region (GRF661611, GRF662413 and 16101015) to PY Qian, as well as grants from the Hundred Talent Program (SIDSSE-BR-201304) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SIDSSE-201304) to LS He.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1002/pmic.201500112

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title = "Nitric oxide inhibits larval settlement in Amphibalanus amphitrite cyprids by repressing muscle locomotion and molting",

abstract = "Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/NO (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecular mechanism, we analyzed changes in the proteome of A. amphitrite cyprids in response to different concentrations of the NO donor sodium nitroprusside (SNP; 62.5, 250 and 1000 μM) using a label-free proteomics method. Compared with the control, the expression of 106 proteins differed in all three treatments. These differentially expressed proteins were assigned to 13 pathways based on KEGG pathway enrichment analysis. SNP treatment stimulated the expression of heat shock proteins and arginine kinase, which are functionally related to NO synthases, increased the expression levels of glutathione transferases for detoxification, and activated the iron-mediated fatty acid degradation pathway and the citrate cycle through ferritin. Moreover, NO repressed the level of myosins and cuticular proteins, which indicated that NO might inhibit larval settlement in A. amphitrite by modulating the process of muscle locomotion and molting.",

author = "Gen Zhang and Yue-Him Wong and Yu Zhang and Li-sheng He and Ying Xu and Pei-Yuan Qian",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by grants from the China Ocean Mineral Resources Research and Development Association (DY125-15-T-02) and the Research Grants Council of the Hong Kong Special Administrative Region (GRF661611, GRF662413 and 16101015) to PY Qian, as well as grants from the Hundred Talent Program (SIDSSE-BR-201304) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SIDSSE-201304) to LS He. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

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AU - Wong, Yue-Him

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AU - He, Li-sheng

AU - Xu, Ying

AU - Qian, Pei-Yuan

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by grants from the China Ocean Mineral Resources Research and Development Association (DY125-15-T-02) and the Research Grants Council of the Hong Kong Special Administrative Region (GRF661611, GRF662413 and 16101015) to PY Qian, as well as grants from the Hundred Talent Program (SIDSSE-BR-201304) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SIDSSE-201304) to LS He. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2015/10/2

Y1 - 2015/10/2

N2 - Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/NO (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecular mechanism, we analyzed changes in the proteome of A. amphitrite cyprids in response to different concentrations of the NO donor sodium nitroprusside (SNP; 62.5, 250 and 1000 μM) using a label-free proteomics method. Compared with the control, the expression of 106 proteins differed in all three treatments. These differentially expressed proteins were assigned to 13 pathways based on KEGG pathway enrichment analysis. SNP treatment stimulated the expression of heat shock proteins and arginine kinase, which are functionally related to NO synthases, increased the expression levels of glutathione transferases for detoxification, and activated the iron-mediated fatty acid degradation pathway and the citrate cycle through ferritin. Moreover, NO repressed the level of myosins and cuticular proteins, which indicated that NO might inhibit larval settlement in A. amphitrite by modulating the process of muscle locomotion and molting.

AB - Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/NO (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecular mechanism, we analyzed changes in the proteome of A. amphitrite cyprids in response to different concentrations of the NO donor sodium nitroprusside (SNP; 62.5, 250 and 1000 μM) using a label-free proteomics method. Compared with the control, the expression of 106 proteins differed in all three treatments. These differentially expressed proteins were assigned to 13 pathways based on KEGG pathway enrichment analysis. SNP treatment stimulated the expression of heat shock proteins and arginine kinase, which are functionally related to NO synthases, increased the expression levels of glutathione transferases for detoxification, and activated the iron-mediated fatty acid degradation pathway and the citrate cycle through ferritin. Moreover, NO repressed the level of myosins and cuticular proteins, which indicated that NO might inhibit larval settlement in A. amphitrite by modulating the process of muscle locomotion and molting.

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Nitric oxide inhibits larval settlement in Amphibalanus amphitrite cyprids by repressing muscle locomotion and molting

Abstract

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