NIRVANA for Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and Co-infections of Multiple Respiratory Viruses

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Detection and mutation surveillance of SARS-CoV-2 are crucial for combating the COVID-19 pandemic. Here we describe a lab-based method for multiplex isothermal amplification-based sequencing and real-time analysis of multiple viral genomes. It can simultaneously detect SARS-CoV-2, influenza A, human adenovirus, and human coronavirus and monitor mutations for up to 96 samples in real time. The method proved to be rapid and sensitive (limit of detection: 29 viral RNA copies/μL of extracted nucleic acid) in detecting SARS-CoV-2 in clinical samples. We expect it to offer a promising solution for rapid field-deployable detection and mutational surveillance of pandemic viruses.
Original languageEnglish (US)
Title of host publicationMultiplex Biomarker Techniques
PublisherSpringer US
Pages79-88
Number of pages10
ISBN (Print)9781071623947
DOIs
StatePublished - Jul 16 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-09-14

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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