NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles

Antonio Buonerba; Rosita Lapenta; Anna Donniacuo; Magda Licasale; Elena Vezzoli; Stefano Milione; Carmine Capacchione; Mario Felice Tecce; Andrea Falqui; Roberto Piacentini; Claudio Grassi; Alfonso Grassi

doi:10.1038/s41598-020-68397-1

NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles

Antonio Buonerba, Rosita Lapenta, Anna Donniacuo, Magda Licasale, Elena Vezzoli, Stefano Milione, Carmine Capacchione, Mario Felice Tecce, Andrea Falqui, Roberto Piacentini, Claudio Grassi, Alfonso Grassi

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Abstract

Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect.

Original language	English (US)
Journal	Scientific Reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-68397-1
State	Published - Jul 9 2020

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: Financial support is acknowledged from: the Ministero dell'Istruzione, dell'Università e della Ricerca (Progetti di Rilevante Interesse Nazionale PRIN 2017), the Università degli Studi di Salerno (FARB 2017), the SPRING cluster (REBIOCHEM research project CTN01_00063_49393), the Regione Campania for the FT-ICR mass spectrometer facility (POR CAMPANIA FESR 2007/2013 O.O.2.1, CUP B46D14002660009), the Farma-BioNet (CUP B25C13000230007) and the Centro di Tecnologie Integrate per la Salute for the 600 MHz NMR facility (CITIS, project PONa3_00138).

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10.1038/s41598-020-68397-1

https://repository.kaust.edu.sa/bitstream/10754/664134/1/Articlefile1.pdf

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Buonerba, A., Lapenta, R., Donniacuo, A., Licasale, M., Vezzoli, E., Milione, S., Capacchione, C., Tecce, M. F., Falqui, A., Piacentini, R., Grassi, C., & Grassi, A. (2020). NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-68397-1

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title = "NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles",

abstract = "Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect.",

author = "Antonio Buonerba and Rosita Lapenta and Anna Donniacuo and Magda Licasale and Elena Vezzoli and Stefano Milione and Carmine Capacchione and Tecce, {Mario Felice} and Andrea Falqui and Roberto Piacentini and Claudio Grassi and Alfonso Grassi",

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Buonerba, A, Lapenta, R, Donniacuo, A, Licasale, M, Vezzoli, E, Milione, S, Capacchione, C, Tecce, MF, Falqui, A, Piacentini, R, Grassi, C & Grassi, A 2020, 'NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles', Scientific Reports, vol. 10, no. 1. https://doi.org/10.1038/s41598-020-68397-1

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T1 - NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles

AU - Buonerba, Antonio

AU - Lapenta, Rosita

AU - Donniacuo, Anna

AU - Licasale, Magda

AU - Vezzoli, Elena

AU - Milione, Stefano

AU - Capacchione, Carmine

AU - Tecce, Mario Felice

AU - Falqui, Andrea

AU - Piacentini, Roberto

AU - Grassi, Claudio

AU - Grassi, Alfonso

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Financial support is acknowledged from: the Ministero dell'Istruzione, dell'Università e della Ricerca (Progetti di Rilevante Interesse Nazionale PRIN 2017), the Università degli Studi di Salerno (FARB 2017), the SPRING cluster (REBIOCHEM research project CTN01_00063_49393), the Regione Campania for the FT-ICR mass spectrometer facility (POR CAMPANIA FESR 2007/2013 O.O.2.1, CUP B46D14002660009), the Farma-BioNet (CUP B25C13000230007) and the Centro di Tecnologie Integrate per la Salute for the 600 MHz NMR facility (CITIS, project PONa3_00138).

PY - 2020/7/9

Y1 - 2020/7/9

N2 - Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect.

AB - Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect.

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JO - Scientific Reports

JF - Scientific Reports

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ER -

NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles

Abstract

Bibliographical note

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