New group-specific 16S rDNA primers for monitoring foaming mycolata during saline waste-water treatment

L. A.I. De Azeredo; C. D. Da Cunha; A. S. Rosado; A. Macrae; D. M.G. Freire; L. C.S. Mendonça-Hagler; G. L. Sant'Anna

doi:10.1007/s10529-005-6180-1

New group-specific 16S rDNA primers for monitoring foaming mycolata during saline waste-water treatment

L. A.I. De Azeredo, C. D. Da Cunha, A. S. Rosado, A. Macrae, D. M.G. Freire, L. C.S. Mendonça-Hagler, G. L. Sant'Anna

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7 Scopus citations

Abstract

Newly designed group-specific PCR primers for denaturing gradient gel electrophoresis (DGGE) were used to investigate foaming mycolata from a bioreactor treating an industrial saline waste-water. Genetic profiles on DGGE gels were different with NaCl at 1.65 and 8.24 g l-1, demonstrating that mycolata community was affected by salinity. A semi-nested PCR strategy resulted in more bands in community genetic profiles than direct amplification. DNA sequencing of bands confirmed the efficacy of the novel primers with sequences recovered being most similar to foam producing mycolata. The new group-specific primers/DGGE approach is a new step toward a more complete understanding of functionally important groups of bacteria involved in biological treatment of waste-water. © Springer 2006.

Original language	English (US)
Pages (from-to)	447-453
Number of pages	7
Journal	Biotechnology Letters
Volume	28
Issue number	6
DOIs	https://doi.org/10.1007/s10529-005-6180-1
State	Published - Mar 1 2006
Externally published	Yes

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title = "New group-specific 16S rDNA primers for monitoring foaming mycolata during saline waste-water treatment",

abstract = "Newly designed group-specific PCR primers for denaturing gradient gel electrophoresis (DGGE) were used to investigate foaming mycolata from a bioreactor treating an industrial saline waste-water. Genetic profiles on DGGE gels were different with NaCl at 1.65 and 8.24 g l-1, demonstrating that mycolata community was affected by salinity. A semi-nested PCR strategy resulted in more bands in community genetic profiles than direct amplification. DNA sequencing of bands confirmed the efficacy of the novel primers with sequences recovered being most similar to foam producing mycolata. The new group-specific primers/DGGE approach is a new step toward a more complete understanding of functionally important groups of bacteria involved in biological treatment of waste-water. {\textcopyright} Springer 2006.",

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doi = "10.1007/s10529-005-6180-1",

language = "English (US)",

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AU - De Azeredo, L. A.I.

AU - Da Cunha, C. D.

AU - Rosado, A. S.

AU - Macrae, A.

AU - Freire, D. M.G.

AU - Mendonça-Hagler, L. C.S.

AU - Sant'Anna, G. L.

N1 - Generated from Scopus record by KAUST IRTS on 2021-02-16

PY - 2006/3/1

Y1 - 2006/3/1

N2 - Newly designed group-specific PCR primers for denaturing gradient gel electrophoresis (DGGE) were used to investigate foaming mycolata from a bioreactor treating an industrial saline waste-water. Genetic profiles on DGGE gels were different with NaCl at 1.65 and 8.24 g l-1, demonstrating that mycolata community was affected by salinity. A semi-nested PCR strategy resulted in more bands in community genetic profiles than direct amplification. DNA sequencing of bands confirmed the efficacy of the novel primers with sequences recovered being most similar to foam producing mycolata. The new group-specific primers/DGGE approach is a new step toward a more complete understanding of functionally important groups of bacteria involved in biological treatment of waste-water. © Springer 2006.

AB - Newly designed group-specific PCR primers for denaturing gradient gel electrophoresis (DGGE) were used to investigate foaming mycolata from a bioreactor treating an industrial saline waste-water. Genetic profiles on DGGE gels were different with NaCl at 1.65 and 8.24 g l-1, demonstrating that mycolata community was affected by salinity. A semi-nested PCR strategy resulted in more bands in community genetic profiles than direct amplification. DNA sequencing of bands confirmed the efficacy of the novel primers with sequences recovered being most similar to foam producing mycolata. The new group-specific primers/DGGE approach is a new step toward a more complete understanding of functionally important groups of bacteria involved in biological treatment of waste-water. © Springer 2006.

UR - http://link.springer.com/10.1007/s10529-005-6180-1

UR - http://www.scopus.com/inward/record.url?scp=33645886626&partnerID=8YFLogxK

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DO - 10.1007/s10529-005-6180-1

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VL - 28

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EP - 453

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 6

ER -

New group-specific 16S rDNA primers for monitoring foaming mycolata during saline waste-water treatment

Abstract

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