Nasopharyngeal Protein Biomarkers of Acute Respiratory Virus Infection

Thomas W. Burke, Ricardo Henao, Erik Soderblom, Ephraim L. Tsalik, J. Will Thompson, Micah T. McClain, Marshall Nichols, Bradly P. Nicholson, Timothy Veldman, Joseph E. Lucas, M. Arthur Moseley, Ronald B. Turner, Robert Lambkin-Williams, Alfred O. Hero, Christopher W. Woods, Geoffrey S. Ginsburg

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Infection of respiratory mucosa with viral pathogens triggers complex immunologic events in the affected host. We sought to characterize this response through proteomic analysis of nasopharyngeal lavage in human subjects experimentally challenged with influenza A/H3N2 or human rhinovirus, and to develop targeted assays measuring peptides involved in this host response allowing classification of acute respiratory virus infection. Unbiased proteomic discovery analysis identified 3285 peptides corresponding to 438 unique proteins, and revealed that infection with H3N2 induces significant alterations in protein expression. These include proteins involved in acute inflammatory response, innate immune response, and the complement cascade. These data provide insights into the nature of the biological response to viral infection of the upper respiratory tract, and the proteins that are dysregulated by viral infection form the basis of signature that accurately classifies the infected state. Verification of this signature using targeted mass spectrometry in independent cohorts of subjects challenged with influenza or rhinovirus demonstrates that it performs with high accuracy (0.8623 AUROC, 75% TPR, 97.46% TNR). With further development as a clinical diagnostic, this signature may have utility in rapid screening for emerging infections, avoidance of inappropriate antibacterial therapy, and more rapid implementation of appropriate therapeutic and public health strategies.
Original languageEnglish (US)
Pages (from-to)172-181
Number of pages10
JournalEBioMedicine
Volume17
DOIs
StatePublished - Mar 1 2017
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-15

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Medicine

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