Narrow Precursor Mass Range for DIA-MS Enhances Protein Identification and Quantification in Arabidopsis.

Huoming Zhang, Dalila Bensaddek

    Research output: Contribution to journalArticlepeer-review

    1 Scopus citations

    Abstract

    Data independent acquisition–mass spectrometry (DIA–MS) is becoming widely utilised for robust and accurate quantification of samples in quantitative proteomics. Here, we describe the systematic evaluation of the effects of DIA precursor mass range on total protein identification and quantification. We show that a narrow mass range of precursors (~250 m/z) for DIA–MS enables a higher number of protein identifications. Subsequent application of DIA with narrow precursor range (from 400 to 650 m/z) on an Arabidopsis sample with spike-in known proteins identified 34.7% more proteins than in conventional DIA (cDIA) with a wide precursor range of 400–1200 m/z. When combining several DIA–MS analyses with narrow precursor ranges (i.e., 400–650, 650–900 and 900–1200 m/z), we were able to quantify 10,099 protein groups with a median coefficient of variation of
    Original languageEnglish (US)
    Pages (from-to)982
    JournalLife (Basel, Switzerland)
    Volume11
    Issue number9
    DOIs
    StatePublished - Sep 28 2021

    ASJC Scopus subject areas

    • Space and Planetary Science
    • Palaeontology
    • Biochemistry, Genetics and Molecular Biology(all)
    • Ecology, Evolution, Behavior and Systematics

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