Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

Marco Gaspari; Mark Ming Cheng Cheng; Rosa Terracciano; Xuewu Liu; A. Jasper Nijdam; Lisa Vaccari; Enzo Di Fabrizio; Emanuel F. Petricoin; Lance A. Liotta; Giovanni Cuda; Salvatore Venuta; Mauro Ferrari

doi:10.1021/pr050417+

Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

Marco Gaspari^*, Mark Ming Cheng Cheng, Rosa Terracciano, Xuewu Liu, A. Jasper Nijdam, Lisa Vaccari, Enzo Di Fabrizio, Emanuel F. Petricoin, Lance A. Liotta, Giovanni Cuda, Salvatore Venuta, Mauro Ferrari

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

Original language	English (US)
Pages (from-to)	1261-1266
Number of pages	6
Journal	Journal of Proteome Research
Volume	5
Issue number	5
DOIs	https://doi.org/10.1021/pr050417+
State	Published - May 2006
Externally published	Yes

Keywords

Human plasma
MALDI-TOF
Mass spectrometry
Nanoporous silica
Nanoporous silicon
Peptide profiling

ASJC Scopus subject areas

Biochemistry
General Chemistry

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10.1021/pr050417+

Cite this

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title = "Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma",

abstract = "Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.",

keywords = "Human plasma, MALDI-TOF, Mass spectrometry, Nanoporous silica, Nanoporous silicon, Peptide profiling",

author = "Marco Gaspari and Cheng, {Mark Ming Cheng} and Rosa Terracciano and Xuewu Liu and Nijdam, {A. Jasper} and Lisa Vaccari and {Di Fabrizio}, Enzo and Petricoin, {Emanuel F.} and Liotta, {Lance A.} and Giovanni Cuda and Salvatore Venuta and Mauro Ferrari",

year = "2006",

month = may,

doi = "10.1021/pr050417+",

language = "English (US)",

volume = "5",

pages = "1261--1266",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

AU - Gaspari, Marco

AU - Cheng, Mark Ming Cheng

AU - Terracciano, Rosa

AU - Liu, Xuewu

AU - Nijdam, A. Jasper

AU - Vaccari, Lisa

AU - Di Fabrizio, Enzo

AU - Petricoin, Emanuel F.

AU - Liotta, Lance A.

AU - Cuda, Giovanni

AU - Venuta, Salvatore

AU - Ferrari, Mauro

PY - 2006/5

Y1 - 2006/5

N2 - Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

AB - Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

KW - Human plasma

KW - MALDI-TOF

KW - Mass spectrometry

KW - Nanoporous silica

KW - Nanoporous silicon

KW - Peptide profiling

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JO - Journal of Proteome Research

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IS - 5

ER -

Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

Abstract

Keywords

ASJC Scopus subject areas

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