Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

Patrizio Candeloro*, Luca Tirinato, Natalia Malara, Annalisa Fregola, Eudald Casals, Victor Puntes, Gerardo Perozziello, Francesco Gentile, Maria Laura Coluccio, Gobind Das, Carlo Liberale, Francesco De Angelis, Enzo Di Fabrizio

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe 3O4 nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.

Original languageEnglish (US)
Pages (from-to)4402-4408
Number of pages7
Issue number21
StatePublished - Nov 7 2011
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry


Dive into the research topics of 'Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies'. Together they form a unique fingerprint.

Cite this