TY - JOUR
T1 - Nanomechanical DNA resonators for sensing and structural analysis of DNA-ligand complexes
AU - Stassi, Stefano
AU - Marini, Monica
AU - Allione, Marco
AU - Lopatin, Sergei
AU - Marson, Domenico
AU - Laurini, Erik
AU - Pricl, Sabrina
AU - Pirri, Candido Fabrizio
AU - Ricciardi, Carlo
AU - Di Fabrizio, Enzo M.
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): OCRF-2014-CRG, OCRF-2016-CRG
Acknowledgements: The authors acknowledge financial support from King Abdullah University of Science and Technology for OCRF-2014-CRG and OCRF-2016-CRG grants and from Piedmont Region through European Funds for Regional Development (“Food Digital Monitoring” project).
PY - 2019/4/12
Y1 - 2019/4/12
N2 - The effect of direct or indirect binding of intercalant molecules on DNA structure is of fundamental importance in understanding the biological functioning of DNA. Here we report on self-suspended DNA nanobundles as ultrasensitive nanomechanical resonators for structural studies of DNA-ligand complexes. Such vibrating nanostructures represent the smallest mechanical resonator entirely composed of DNA. A correlative analysis between the mechanical and structural properties is exploited to study the intrinsic changes of double strand DNA, when interacting with different intercalant molecules (YOYO-1 and GelRed) and a chemotherapeutic drug (Cisplatin), at different concentrations. Possible implications of our findings are related to the study of interaction mechanism of a wide category of molecules with DNA, and to further applications in medicine, such as optimal titration of chemotherapeutic drugs and environmental studies for the detection of heavy metals in human serum.
AB - The effect of direct or indirect binding of intercalant molecules on DNA structure is of fundamental importance in understanding the biological functioning of DNA. Here we report on self-suspended DNA nanobundles as ultrasensitive nanomechanical resonators for structural studies of DNA-ligand complexes. Such vibrating nanostructures represent the smallest mechanical resonator entirely composed of DNA. A correlative analysis between the mechanical and structural properties is exploited to study the intrinsic changes of double strand DNA, when interacting with different intercalant molecules (YOYO-1 and GelRed) and a chemotherapeutic drug (Cisplatin), at different concentrations. Possible implications of our findings are related to the study of interaction mechanism of a wide category of molecules with DNA, and to further applications in medicine, such as optimal titration of chemotherapeutic drugs and environmental studies for the detection of heavy metals in human serum.
UR - http://hdl.handle.net/10754/631882
UR - https://www.nature.com/articles/s41467-019-09612-0
UR - http://www.scopus.com/inward/record.url?scp=85064346857&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-09612-0
DO - 10.1038/s41467-019-09612-0
M3 - Article
C2 - 30979901
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
ER -
