Nanodecoy system: A novel approach to design hepatitis B vaccine for immunopotentiation

Amit K. Goyal, Amit Rawat, Sunil Mahor, Prem N. Gupta, Kapil Khatri, Suresh P. Vyas

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The progress toward subunit vaccines has been limited by their poor immunogenicity and limited stability. To enhance the immune response, subunit vaccines universally require improved adjuvants and delivery vehicles. In the present study, we propose the use of ceramic core based nanodecoy systems for effective immunization, which seems to exhibit a broad range of surface properties. Nanodecoy systems were prepared by self-assembling of hydroxyapatite core and cellobiose and finally the hepatitis B surface antigen (HBsAg) was adsorbed over the preformed nanodecoy systems. HBsAg loaded nanodecoy systems were characterized for size, shape and antigen loading efficiency. The effect of processing steps on the stability and integrity of HBsAg was assessed by in vitro antigenicity and SDS-PAGE experiments. Nanodecoy preparations were nanometric in size range and almost spherical in shape. SDS-PAGE studies confirmed the integrity of HBsAg protein in the formulation. Vaccine efficacy was determined in female Balb/c mice and results indicated that specific anti-HBsAg antibody titers in mice receiving nanodecoy system were more efficient than the conventional adjuvant alum followed by subcutaneous immunization. Studies also indicated that nanodecoy formulations could elicit combined Th1 and Th2 immune response. It is inferred that nanodecoy systems are a class of novel carriers and hold potential as an alternative adjuvant in vaccine technology. © 2005 Elsevier B.V. All rights reserved.
Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalInternational Journal of Pharmaceutics
Volume309
Issue number1-2
DOIs
StatePublished - Feb 17 2006
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-10-12

ASJC Scopus subject areas

  • Pharmaceutical Science

Fingerprint

Dive into the research topics of 'Nanodecoy system: A novel approach to design hepatitis B vaccine for immunopotentiation'. Together they form a unique fingerprint.

Cite this