Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR

Songlin Wang; Sudhakar Parthasarathy; Yiling Xiao; Yusuke Nishiyama; Fei Long; Isamu Matsuda; Yuki Endo; Takahiro Nemoto; Kazuo Yamauchi; Tetsuo Asakura; Mitsuhiro Takeda; Tsutomu Terauchi; Masatsune Kainosho; Yoshitaka Ishii

doi:10.1039/c5cc04618a

Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR

Songlin Wang, Sudhakar Parthasarathy, Yiling Xiao, Yusuke Nishiyama, Fei Long, Isamu Matsuda, Yuki Endo, Takahiro Nemoto, Kazuo Yamauchi, Tetsuo Asakura, Mitsuhiro Takeda, Tsutomu Terauchi, Masatsune Kainosho, Yoshitaka Ishii

Imaging & Characterization Core Lab

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34 Scopus citations

Abstract

We present a 3D 1H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D 13C-detected SSNMR approach. This journal is © The Royal Society of Chemistry 2015.

Original language	English (US)
Pages (from-to)	15055-15058
Number of pages	4
Journal	Chem. Commun.
Volume	51
Issue number	81
DOIs	https://doi.org/10.1039/c5cc04618a
State	Published - Aug 28 2015

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: National Institute of General Medical Sciences[9R01GM098033]

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10.1039/c5cc04618a

https://repository.kaust.edu.sa/bitstream/10754/594287/1/Articlefile1.pdf

Cite this

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title = "Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR",

abstract = "We present a 3D 1H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D 13C-detected SSNMR approach. This journal is {\textcopyright} The Royal Society of Chemistry 2015.",

author = "Songlin Wang and Sudhakar Parthasarathy and Yiling Xiao and Yusuke Nishiyama and Fei Long and Isamu Matsuda and Yuki Endo and Takahiro Nemoto and Kazuo Yamauchi and Tetsuo Asakura and Mitsuhiro Takeda and Tsutomu Terauchi and Masatsune Kainosho and Yoshitaka Ishii",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: National Institute of General Medical Sciences[9R01GM098033]",

year = "2015",

month = aug,

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doi = "10.1039/c5cc04618a",

language = "English (US)",

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T1 - Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR

AU - Wang, Songlin

AU - Parthasarathy, Sudhakar

AU - Xiao, Yiling

AU - Nishiyama, Yusuke

AU - Long, Fei

AU - Matsuda, Isamu

AU - Endo, Yuki

AU - Nemoto, Takahiro

AU - Yamauchi, Kazuo

AU - Asakura, Tetsuo

AU - Takeda, Mitsuhiro

AU - Terauchi, Tsutomu

AU - Kainosho, Masatsune

AU - Ishii, Yoshitaka

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: National Institute of General Medical Sciences[9R01GM098033]

PY - 2015/8/28

Y1 - 2015/8/28

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AB - We present a 3D 1H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D 13C-detected SSNMR approach. This journal is © The Royal Society of Chemistry 2015.

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EP - 15058

JO - Chem. Commun.

JF - Chem. Commun.

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ER -

Nano-mole scale sequential signal assignment by 1 H-detected protein solid-state NMR

Abstract

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