MYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature

Brecht Wybouw; Helena E Arents; Baojun Yang; Jonah Nolf; Wouter Smet; Michael Vandorpe; Max Minne; Xiaopeng Luo; Inge De Clercq; Daniël Van Damme; Matouš Glanc; Bert De Rybel

doi:10.1093/jxb/erad020

MYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature

Brecht Wybouw, Helena E Arents, Baojun Yang, Jonah Nolf, Wouter Smet, Michael Vandorpe, Max Minne, Xiaopeng Luo, Inge De Clercq, Daniël Van Damme, Matouš Glanc, Bert De Rybel

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Transcriptional networks are crucial to integrate various internal and external signals into optimal responses during plant growth and development. Primary root vasculature patterning and proliferation are controlled by a network centred around the basic Helix-Loop-Helix transcription factor complex formed by TARGET OF MONOPTEROS 5 (TMO5) and LONESOME HIGHWAY (LHW), which control cell proliferation and division orientation by modulating cytokinin response and other downstream factors. Despite recent progress, many aspects of the TMO5/LHW pathway are not fully understood. In particular, the upstream regulators of TMO5/LHW activity remain unknown. Here, using a forward genetic approach to identify new factors of the TMO5/LHW pathway, we discovered a novel function of the MYB-type transcription factor MYB12. MYB12 physically interacts with TMO5 and dampens the TMO5/LHW-mediated induction of direct target gene expression as well as the periclinal/radial cell divisions. The expression of MYB12 is activated by the cytokinin response, downstream of TMO5/LHW, resulting in a novel MYB12-mediated negative feedback loop that restricts TMO5/LHW activity to ensure optimal cell proliferation rates during root vascular development.

Original language	English (US)
Journal	Journal of Experimental Botany
DOIs	https://doi.org/10.1093/jxb/erad020
State	Published - Jan 18 2023

Bibliographical note

KAUST Repository Item: Exported on 2023-01-20
Acknowledgements: This work was funded by The Research Foundation - Flanders (FWO; Odysseus II G0D0515N and post-doc fellowship 1215820N); the Netherlands Organization for Scientific Research (NWO; VIDI 864.13.00); the European Research Council (ERC Starting Grant TORPEDO; 714055); the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 885979 “DIVISION BELL”; EMBO (long term fellowship ALTF 1005-2019); and ERC consolidator Grant T-REX; 682436 to DVD; ERC Starting Grant COSI; 949808 to I.D.C and the China Scholarship Council (PhD fellowship 201706910099) to X.L. The authors thank Ralf Stracke and Sofie Goormachtig for sharing the myb11myb12-1f,myb111 and myb12-f mutant seeds, respectively; Davy Opdenacker for help with the EMS mutagenesis and Frederik Coppens for help with the SHOREmap analysis. B.D.R. would like to thank T.G.A. and A.P.M. for helpful discussions completely unrelated to this work.

ASJC Scopus subject areas

Plant Science
Physiology

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10.1093/jxb/erad020

https://repository.kaust.edu.sa/bitstream/10754/687152/1/erad020.pdf

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@article{0723e484400444d4860d7e4bf12e7808,

title = "MYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature",

abstract = "Transcriptional networks are crucial to integrate various internal and external signals into optimal responses during plant growth and development. Primary root vasculature patterning and proliferation are controlled by a network centred around the basic Helix-Loop-Helix transcription factor complex formed by TARGET OF MONOPTEROS 5 (TMO5) and LONESOME HIGHWAY (LHW), which control cell proliferation and division orientation by modulating cytokinin response and other downstream factors. Despite recent progress, many aspects of the TMO5/LHW pathway are not fully understood. In particular, the upstream regulators of TMO5/LHW activity remain unknown. Here, using a forward genetic approach to identify new factors of the TMO5/LHW pathway, we discovered a novel function of the MYB-type transcription factor MYB12. MYB12 physically interacts with TMO5 and dampens the TMO5/LHW-mediated induction of direct target gene expression as well as the periclinal/radial cell divisions. The expression of MYB12 is activated by the cytokinin response, downstream of TMO5/LHW, resulting in a novel MYB12-mediated negative feedback loop that restricts TMO5/LHW activity to ensure optimal cell proliferation rates during root vascular development.",

author = "Brecht Wybouw and Arents, {Helena E} and Baojun Yang and Jonah Nolf and Wouter Smet and Michael Vandorpe and Max Minne and Xiaopeng Luo and {De Clercq}, Inge and {Van Damme}, Dani{\"e}l and Matou{\v s} Glanc and {De Rybel}, Bert",

note = "KAUST Repository Item: Exported on 2023-01-20 Acknowledgements: This work was funded by The Research Foundation - Flanders (FWO; Odysseus II G0D0515N and post-doc fellowship 1215820N); the Netherlands Organization for Scientific Research (NWO; VIDI 864.13.00); the European Research Council (ERC Starting Grant TORPEDO; 714055); the European Union{\textquoteright}s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 885979 “DIVISION BELL”; EMBO (long term fellowship ALTF 1005-2019); and ERC consolidator Grant T-REX; 682436 to DVD; ERC Starting Grant COSI; 949808 to I.D.C and the China Scholarship Council (PhD fellowship 201706910099) to X.L. The authors thank Ralf Stracke and Sofie Goormachtig for sharing the myb11myb12-1f,myb111 and myb12-f mutant seeds, respectively; Davy Opdenacker for help with the EMS mutagenesis and Frederik Coppens for help with the SHOREmap analysis. B.D.R. would like to thank T.G.A. and A.P.M. for helpful discussions completely unrelated to this work.",

year = "2023",

month = jan,

day = "18",

doi = "10.1093/jxb/erad020",

language = "English (US)",

journal = "Journal of Experimental Botany",

issn = "0022-0957",

publisher = "OXFORD UNIV PRESS",

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TY - JOUR

T1 - MYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature

AU - Wybouw, Brecht

AU - Arents, Helena E

AU - Yang, Baojun

AU - Nolf, Jonah

AU - Smet, Wouter

AU - Vandorpe, Michael

AU - Minne, Max

AU - Luo, Xiaopeng

AU - De Clercq, Inge

AU - Van Damme, Daniël

AU - Glanc, Matouš

AU - De Rybel, Bert

N1 - KAUST Repository Item: Exported on 2023-01-20 Acknowledgements: This work was funded by The Research Foundation - Flanders (FWO; Odysseus II G0D0515N and post-doc fellowship 1215820N); the Netherlands Organization for Scientific Research (NWO; VIDI 864.13.00); the European Research Council (ERC Starting Grant TORPEDO; 714055); the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 885979 “DIVISION BELL”; EMBO (long term fellowship ALTF 1005-2019); and ERC consolidator Grant T-REX; 682436 to DVD; ERC Starting Grant COSI; 949808 to I.D.C and the China Scholarship Council (PhD fellowship 201706910099) to X.L. The authors thank Ralf Stracke and Sofie Goormachtig for sharing the myb11myb12-1f,myb111 and myb12-f mutant seeds, respectively; Davy Opdenacker for help with the EMS mutagenesis and Frederik Coppens for help with the SHOREmap analysis. B.D.R. would like to thank T.G.A. and A.P.M. for helpful discussions completely unrelated to this work.

PY - 2023/1/18

Y1 - 2023/1/18

N2 - Transcriptional networks are crucial to integrate various internal and external signals into optimal responses during plant growth and development. Primary root vasculature patterning and proliferation are controlled by a network centred around the basic Helix-Loop-Helix transcription factor complex formed by TARGET OF MONOPTEROS 5 (TMO5) and LONESOME HIGHWAY (LHW), which control cell proliferation and division orientation by modulating cytokinin response and other downstream factors. Despite recent progress, many aspects of the TMO5/LHW pathway are not fully understood. In particular, the upstream regulators of TMO5/LHW activity remain unknown. Here, using a forward genetic approach to identify new factors of the TMO5/LHW pathway, we discovered a novel function of the MYB-type transcription factor MYB12. MYB12 physically interacts with TMO5 and dampens the TMO5/LHW-mediated induction of direct target gene expression as well as the periclinal/radial cell divisions. The expression of MYB12 is activated by the cytokinin response, downstream of TMO5/LHW, resulting in a novel MYB12-mediated negative feedback loop that restricts TMO5/LHW activity to ensure optimal cell proliferation rates during root vascular development.

AB - Transcriptional networks are crucial to integrate various internal and external signals into optimal responses during plant growth and development. Primary root vasculature patterning and proliferation are controlled by a network centred around the basic Helix-Loop-Helix transcription factor complex formed by TARGET OF MONOPTEROS 5 (TMO5) and LONESOME HIGHWAY (LHW), which control cell proliferation and division orientation by modulating cytokinin response and other downstream factors. Despite recent progress, many aspects of the TMO5/LHW pathway are not fully understood. In particular, the upstream regulators of TMO5/LHW activity remain unknown. Here, using a forward genetic approach to identify new factors of the TMO5/LHW pathway, we discovered a novel function of the MYB-type transcription factor MYB12. MYB12 physically interacts with TMO5 and dampens the TMO5/LHW-mediated induction of direct target gene expression as well as the periclinal/radial cell divisions. The expression of MYB12 is activated by the cytokinin response, downstream of TMO5/LHW, resulting in a novel MYB12-mediated negative feedback loop that restricts TMO5/LHW activity to ensure optimal cell proliferation rates during root vascular development.

UR - http://hdl.handle.net/10754/687152

UR - https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erad020/6991119

U2 - 10.1093/jxb/erad020

DO - 10.1093/jxb/erad020

M3 - Article

C2 - 36651677

SN - 0022-0957

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

ER -

MYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature

Abstract

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