Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique

Satoshi Nagai; Hungyen Chen; Yoko Kawakami; Keigo Yamamoto; Sirje Sildever; Nanako Kanno; Hiroshi Oikawa; Motoshige Yasuike; Yoji Nakamura; Yuki Hongo; Atushi Fujiwara; Takanori Kobayashi; Takashi Gojobori

doi:10.1016/j.hal.2019.101660

Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique

Satoshi Nagai, Hungyen Chen, Yoko Kawakami, Keigo Yamamoto, Sirje Sildever, Nanako Kanno, Hiroshi Oikawa, Motoshige Yasuike, Yoji Nakamura, Yuki Hongo, Atushi Fujiwara, Takanori Kobayashi, Takashi Gojobori

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Abstract

Since 2002, blooms of Alexandrium catenella sensu Fraga et al. (2015) and paralytic shellfish toxicity events have occurred almost yearly in Osaka Bay, Japan. To better understand the triggers for reoccurring A. catenella blooms in Osaka Bay, phytoplankton community was monitored during the spring seasons of 2012–2015. Monitoring was performed using massively parallel sequencing (MPS)-based technique on amplicon sequences of the 18S rRNA gene. Dense blooms of A. catenella occurred every year except in 2012, however, there was no significant correlation with the environmental parameters investigated. Plankton community diversity decreased before and middle of the A. catenella blooms, suggesting that the decline in diversity could be an indicator for the bloom occurrence. The yearly abundance pattern of A. catenella cells obtained by morphology-based counting coincided with the relative sequence abundances, which supports the effectiveness of MPS-based phytoplankton monitoring.

Original language	English (US)
Pages (from-to)	101660
Journal	Harmful Algae
Volume	89
DOIs	https://doi.org/10.1016/j.hal.2019.101660
State	Published - Sep 12 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank Dr. Kyoko Yarimizu, National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, for critical reading of the manuscript and encouragement during this study. A. Kondo and R. Kubota are thanked for their help with the molecular work. This work was supported by a Grant-in-Aid (Marine Metagenomics for Monitoring the Coastal Microbiota) by the Ministry of Agriculture, Forestry, and Fisheries of Japan; a Grant-in-Aid for Scientiﬁc Research (Kiban-B) by the Japan Society for the Promotion of Science (Grant number:25292130); and a Grant-in-Aid by the Japanese Society for the Promotion of Science Fellows (Grant number: 02523180 and PE18028). [CG]

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10.1016/j.hal.2019.101660

https://repository.kaust.edu.sa/bitstream/10754/658608/1/1-s2.0-S1568988319301337-main.pdf

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Nagai, S., Chen, H., Kawakami, Y., Yamamoto, K., Sildever, S., Kanno, N., Oikawa, H., Yasuike, M., Nakamura, Y., Hongo, Y., Fujiwara, A., Kobayashi, T., & Gojobori, T. (2019). Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique. Harmful Algae, 89, 101660. https://doi.org/10.1016/j.hal.2019.101660

@article{8586846708c3443589177cbc1377755c,

title = "Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique",

abstract = "Since 2002, blooms of Alexandrium catenella sensu Fraga et al. (2015) and paralytic shellfish toxicity events have occurred almost yearly in Osaka Bay, Japan. To better understand the triggers for reoccurring A. catenella blooms in Osaka Bay, phytoplankton community was monitored during the spring seasons of 2012–2015. Monitoring was performed using massively parallel sequencing (MPS)-based technique on amplicon sequences of the 18S rRNA gene. Dense blooms of A. catenella occurred every year except in 2012, however, there was no significant correlation with the environmental parameters investigated. Plankton community diversity decreased before and middle of the A. catenella blooms, suggesting that the decline in diversity could be an indicator for the bloom occurrence. The yearly abundance pattern of A. catenella cells obtained by morphology-based counting coincided with the relative sequence abundances, which supports the effectiveness of MPS-based phytoplankton monitoring.",

author = "Satoshi Nagai and Hungyen Chen and Yoko Kawakami and Keigo Yamamoto and Sirje Sildever and Nanako Kanno and Hiroshi Oikawa and Motoshige Yasuike and Yoji Nakamura and Yuki Hongo and Atushi Fujiwara and Takanori Kobayashi and Takashi Gojobori",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We thank Dr. Kyoko Yarimizu, National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, for critical reading of the manuscript and encouragement during this study. A. Kondo and R. Kubota are thanked for their help with the molecular work. This work was supported by a Grant-in-Aid (Marine Metagenomics for Monitoring the Coastal Microbiota) by the Ministry of Agriculture, Forestry, and Fisheries of Japan; a Grant-in-Aid for Scientiﬁc Research (Kiban-B) by the Japan Society for the Promotion of Science (Grant number:25292130); and a Grant-in-Aid by the Japanese Society for the Promotion of Science Fellows (Grant number: 02523180 and PE18028). [CG]",

year = "2019",

month = sep,

day = "12",

doi = "10.1016/j.hal.2019.101660",

language = "English (US)",

volume = "89",

pages = "101660",

journal = "Harmful Algae",

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Nagai, S, Chen, H, Kawakami, Y, Yamamoto, K, Sildever, S, Kanno, N, Oikawa, H, Yasuike, M, Nakamura, Y, Hongo, Y, Fujiwara, A, Kobayashi, T & Gojobori, T 2019, 'Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique', Harmful Algae, vol. 89, pp. 101660. https://doi.org/10.1016/j.hal.2019.101660

TY - JOUR

T1 - Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique

AU - Nagai, Satoshi

AU - Chen, Hungyen

AU - Kawakami, Yoko

AU - Yamamoto, Keigo

AU - Sildever, Sirje

AU - Kanno, Nanako

AU - Oikawa, Hiroshi

AU - Yasuike, Motoshige

AU - Nakamura, Yoji

AU - Hongo, Yuki

AU - Fujiwara, Atushi

AU - Kobayashi, Takanori

AU - Gojobori, Takashi

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We thank Dr. Kyoko Yarimizu, National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, for critical reading of the manuscript and encouragement during this study. A. Kondo and R. Kubota are thanked for their help with the molecular work. This work was supported by a Grant-in-Aid (Marine Metagenomics for Monitoring the Coastal Microbiota) by the Ministry of Agriculture, Forestry, and Fisheries of Japan; a Grant-in-Aid for Scientiﬁc Research (Kiban-B) by the Japan Society for the Promotion of Science (Grant number:25292130); and a Grant-in-Aid by the Japanese Society for the Promotion of Science Fellows (Grant number: 02523180 and PE18028). [CG]

PY - 2019/9/12

Y1 - 2019/9/12

N2 - Since 2002, blooms of Alexandrium catenella sensu Fraga et al. (2015) and paralytic shellfish toxicity events have occurred almost yearly in Osaka Bay, Japan. To better understand the triggers for reoccurring A. catenella blooms in Osaka Bay, phytoplankton community was monitored during the spring seasons of 2012–2015. Monitoring was performed using massively parallel sequencing (MPS)-based technique on amplicon sequences of the 18S rRNA gene. Dense blooms of A. catenella occurred every year except in 2012, however, there was no significant correlation with the environmental parameters investigated. Plankton community diversity decreased before and middle of the A. catenella blooms, suggesting that the decline in diversity could be an indicator for the bloom occurrence. The yearly abundance pattern of A. catenella cells obtained by morphology-based counting coincided with the relative sequence abundances, which supports the effectiveness of MPS-based phytoplankton monitoring.

AB - Since 2002, blooms of Alexandrium catenella sensu Fraga et al. (2015) and paralytic shellfish toxicity events have occurred almost yearly in Osaka Bay, Japan. To better understand the triggers for reoccurring A. catenella blooms in Osaka Bay, phytoplankton community was monitored during the spring seasons of 2012–2015. Monitoring was performed using massively parallel sequencing (MPS)-based technique on amplicon sequences of the 18S rRNA gene. Dense blooms of A. catenella occurred every year except in 2012, however, there was no significant correlation with the environmental parameters investigated. Plankton community diversity decreased before and middle of the A. catenella blooms, suggesting that the decline in diversity could be an indicator for the bloom occurrence. The yearly abundance pattern of A. catenella cells obtained by morphology-based counting coincided with the relative sequence abundances, which supports the effectiveness of MPS-based phytoplankton monitoring.

UR - http://hdl.handle.net/10754/658608

UR - https://linkinghub.elsevier.com/retrieve/pii/S1568988319301337

UR - http://www.scopus.com/inward/record.url?scp=85072223415&partnerID=8YFLogxK

U2 - 10.1016/j.hal.2019.101660

DO - 10.1016/j.hal.2019.101660

M3 - Article

C2 - 31672234

SN - 1568-9883

VL - 89

SP - 101660

JO - Harmful Algae

JF - Harmful Algae

ER -

Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique

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