Molecular evolutionary analyses implicate injection treatment for schistosomiasis in the initial hepatitis C epidemics in Japan

Yasuhito Tanaka; Kousuke Hanada; Etsuro Orito; Yoshihiro Akahane; Kazuaki Chayama; Hiroshi Yoshizawa; Michio Sata; Nobuo Ohta; Yuzo Miyakawa; Takashi Gojobori; Masashi Mizokami

doi:10.1016/j.jhep.2004.09.023

Molecular evolutionary analyses implicate injection treatment for schistosomiasis in the initial hepatitis C epidemics in Japan

Yasuhito Tanaka, Kousuke Hanada, Etsuro Orito, Yoshihiro Akahane, Kazuaki Chayama, Hiroshi Yoshizawa, Michio Sata, Nobuo Ohta, Yuzo Miyakawa, Takashi Gojobori, Masashi Mizokami

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40 Scopus citations

Abstract

The mortality due to hepatocellular carcinoma (HCC) has ranged widely in various areas of Japan since 30 years ago and the incidence was particularly high in once Schistosoma japonicum (Sj)-endemic areas. Our aim was to estimate the spread time of hepatitis C virus (HCV) infection in the past with possible relevance to a higher incidence of HCC in once Sj-endemic than Sj-nonendemic areas. During 2001, 131 strains of HCV-1b were obtained from patients in three previously Sj-endemic areas, as well as Sj-nonendemic areas in Japan and a cross-sectional study was conducted on them with molecular evolutionary analyses. A phylogenetic tree reconstructed on HCV-1b sequences in the NS5B region disclosed 2 independent clusters for Sj-positive and -negative groups with a high bootstrap value. The estimated effective number of HCV-infections indicated a transition from quiescence to rapid exponential growth in the 1920s among patients with schistosomiasis, which is 20 years earlier than that among patients without schistosomiasis. The estimated spread time in previously Sj-endemic areas in Japan coincides with injection treatment for Sj since 1921. A high incidence of HCC there would be attributed to a long duration of HCV infection since 1920s.

Original language	English (US)
Pages (from-to)	47-53
Number of pages	7
Journal	Journal of Hepatology
Volume	42
Issue number	1
DOIs	https://doi.org/10.1016/j.jhep.2004.09.023
State	Published - Jan 2005
Externally published	Yes

Bibliographical note

Funding Information:
We greatly appreciate Dr Oliver G. Pybus (Department of Zoology, University of Oxford, Oxford, UK) for his enlightening advice on molecular evolutionary analyses using Genie v3.5. We thank Ms Yoshiko Kobayashi (Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan) for technical assistance and Dr Tatsuya Ide (Kurume University School of Medicine, Fukuoka, Japan) and Dr Hirotaka Kono (Hiroshima University Graduate School of Biomedical Sciences, School of Medicine, Hiroshima, Japan) for providing us with valuable materials. The work was supported by a grant-in-aid from the Ministry of Health, Labour and Welfare of Japan (H13-kanen-2), grants-in-aid for Young Scientists (A) from the Ministry of Education, Culture, Science and Sports of Japan (16689016), The Hori Information Science Promotion Foundation and Miyakawa Memorial Research Foundation.

Keywords

Hepatitis C virus
Hepatocellular carcinoma
Molecular evolutionary analysis
Schistosoma japonicum

ASJC Scopus subject areas

Hepatology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jhep.2004.09.023

Cite this

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title = "Molecular evolutionary analyses implicate injection treatment for schistosomiasis in the initial hepatitis C epidemics in Japan",

abstract = "The mortality due to hepatocellular carcinoma (HCC) has ranged widely in various areas of Japan since 30 years ago and the incidence was particularly high in once Schistosoma japonicum (Sj)-endemic areas. Our aim was to estimate the spread time of hepatitis C virus (HCV) infection in the past with possible relevance to a higher incidence of HCC in once Sj-endemic than Sj-nonendemic areas. During 2001, 131 strains of HCV-1b were obtained from patients in three previously Sj-endemic areas, as well as Sj-nonendemic areas in Japan and a cross-sectional study was conducted on them with molecular evolutionary analyses. A phylogenetic tree reconstructed on HCV-1b sequences in the NS5B region disclosed 2 independent clusters for Sj-positive and -negative groups with a high bootstrap value. The estimated effective number of HCV-infections indicated a transition from quiescence to rapid exponential growth in the 1920s among patients with schistosomiasis, which is 20 years earlier than that among patients without schistosomiasis. The estimated spread time in previously Sj-endemic areas in Japan coincides with injection treatment for Sj since 1921. A high incidence of HCC there would be attributed to a long duration of HCV infection since 1920s.",

keywords = "Hepatitis C virus, Hepatocellular carcinoma, Molecular evolutionary analysis, Schistosoma japonicum",

author = "Yasuhito Tanaka and Kousuke Hanada and Etsuro Orito and Yoshihiro Akahane and Kazuaki Chayama and Hiroshi Yoshizawa and Michio Sata and Nobuo Ohta and Yuzo Miyakawa and Takashi Gojobori and Masashi Mizokami",

note = "Funding Information: We greatly appreciate Dr Oliver G. Pybus (Department of Zoology, University of Oxford, Oxford, UK) for his enlightening advice on molecular evolutionary analyses using Genie v3.5. We thank Ms Yoshiko Kobayashi (Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan) for technical assistance and Dr Tatsuya Ide (Kurume University School of Medicine, Fukuoka, Japan) and Dr Hirotaka Kono (Hiroshima University Graduate School of Biomedical Sciences, School of Medicine, Hiroshima, Japan) for providing us with valuable materials. The work was supported by a grant-in-aid from the Ministry of Health, Labour and Welfare of Japan (H13-kanen-2), grants-in-aid for Young Scientists (A) from the Ministry of Education, Culture, Science and Sports of Japan (16689016), The Hori Information Science Promotion Foundation and Miyakawa Memorial Research Foundation.",

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doi = "10.1016/j.jhep.2004.09.023",

language = "English (US)",

volume = "42",

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AU - Tanaka, Yasuhito

AU - Hanada, Kousuke

AU - Orito, Etsuro

AU - Akahane, Yoshihiro

AU - Chayama, Kazuaki

AU - Yoshizawa, Hiroshi

AU - Sata, Michio

AU - Ohta, Nobuo

AU - Miyakawa, Yuzo

AU - Gojobori, Takashi

AU - Mizokami, Masashi

N1 - Funding Information: We greatly appreciate Dr Oliver G. Pybus (Department of Zoology, University of Oxford, Oxford, UK) for his enlightening advice on molecular evolutionary analyses using Genie v3.5. We thank Ms Yoshiko Kobayashi (Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan) for technical assistance and Dr Tatsuya Ide (Kurume University School of Medicine, Fukuoka, Japan) and Dr Hirotaka Kono (Hiroshima University Graduate School of Biomedical Sciences, School of Medicine, Hiroshima, Japan) for providing us with valuable materials. The work was supported by a grant-in-aid from the Ministry of Health, Labour and Welfare of Japan (H13-kanen-2), grants-in-aid for Young Scientists (A) from the Ministry of Education, Culture, Science and Sports of Japan (16689016), The Hori Information Science Promotion Foundation and Miyakawa Memorial Research Foundation.

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Y1 - 2005/1

N2 - The mortality due to hepatocellular carcinoma (HCC) has ranged widely in various areas of Japan since 30 years ago and the incidence was particularly high in once Schistosoma japonicum (Sj)-endemic areas. Our aim was to estimate the spread time of hepatitis C virus (HCV) infection in the past with possible relevance to a higher incidence of HCC in once Sj-endemic than Sj-nonendemic areas. During 2001, 131 strains of HCV-1b were obtained from patients in three previously Sj-endemic areas, as well as Sj-nonendemic areas in Japan and a cross-sectional study was conducted on them with molecular evolutionary analyses. A phylogenetic tree reconstructed on HCV-1b sequences in the NS5B region disclosed 2 independent clusters for Sj-positive and -negative groups with a high bootstrap value. The estimated effective number of HCV-infections indicated a transition from quiescence to rapid exponential growth in the 1920s among patients with schistosomiasis, which is 20 years earlier than that among patients without schistosomiasis. The estimated spread time in previously Sj-endemic areas in Japan coincides with injection treatment for Sj since 1921. A high incidence of HCC there would be attributed to a long duration of HCV infection since 1920s.

AB - The mortality due to hepatocellular carcinoma (HCC) has ranged widely in various areas of Japan since 30 years ago and the incidence was particularly high in once Schistosoma japonicum (Sj)-endemic areas. Our aim was to estimate the spread time of hepatitis C virus (HCV) infection in the past with possible relevance to a higher incidence of HCC in once Sj-endemic than Sj-nonendemic areas. During 2001, 131 strains of HCV-1b were obtained from patients in three previously Sj-endemic areas, as well as Sj-nonendemic areas in Japan and a cross-sectional study was conducted on them with molecular evolutionary analyses. A phylogenetic tree reconstructed on HCV-1b sequences in the NS5B region disclosed 2 independent clusters for Sj-positive and -negative groups with a high bootstrap value. The estimated effective number of HCV-infections indicated a transition from quiescence to rapid exponential growth in the 1920s among patients with schistosomiasis, which is 20 years earlier than that among patients without schistosomiasis. The estimated spread time in previously Sj-endemic areas in Japan coincides with injection treatment for Sj since 1921. A high incidence of HCC there would be attributed to a long duration of HCV infection since 1920s.

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KW - Hepatocellular carcinoma

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IS - 1

ER -

Molecular evolutionary analyses implicate injection treatment for schistosomiasis in the initial hepatitis C epidemics in Japan

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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