Molecular confirmation of hybridization between Dascyllus reticulatus × Dascyllus aruanus from the Great Barrier Reef

Song He; Jacob L. Johansen; Andrew S. Hoey; Melissa K. Pappas; Michael L. Berumen

doi:10.1007/s12526-017-0819-8

Molecular confirmation of hybridization between Dascyllus reticulatus × Dascyllus aruanus from the Great Barrier Reef

Song He^*, Jacob L. Johansen, Andrew S. Hoey, Melissa K. Pappas, Michael L. Berumen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

To date, more than 81 species of tropical coral reef fish have been reported to hybridize in nature, spanning multiple families, including the Chaetodontidae, Pomacanthidae, and Labridae. Hybridization, however, is seemingly rare among benthic nesting species that engage in pair spawning, such as the Pomacentridae. Here, we present evidence for the first molecularly confirmed record of hybridization within the genus Dascyllus; D. aruanus and D. reticulatus. Interestingly, although many hybridization events are attributed to peripheral range effects or areas of limited overlap among otherwise allopatric species, this hybrid individual was collected from the northern Great Barrier Reef, centrally located within the distribution ranges of both species. The hybrid exhibited coloration and meristic counts intermediate between D. aruanus and D. reticulatus. Diagnostic genetic markers and subsequent microsatellites analysis confirmed that this individual was a hybrid offspring of D. aruanus and D. reticulatus, with the latter providing the maternal contribution. The occurrence of the D. aruanus × D. reticulatus hybrid on the Great Barrier Reef represents an exception to the otherwise species-specific haplotypes. The nuclear diagnostic marker which was identified during this study could serve as a hybrid indicator and benefit future hybrid investigations for hybridization between these two species.

Original language	English (US)
Pages (from-to)	395-404
Number of pages	10
Journal	Marine Biodiversity
Volume	49
Issue number	1
DOIs	https://doi.org/10.1007/s12526-017-0819-8
State	Published - Feb 5 2019
Externally published	Yes

Bibliographical note

Funding Information:
This study was conducted under Great Barrier Reef Marine Park Authority permit G11/34319.1 and QLD Fisheries permit 103256. Ethical approval was obtained from James Cook University (permit A1267). We thank the Lizard Island Research Station staff for fieldwork support and the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory for laboratory support. We thank Gerry Allen, Tane Sinclair-Taylor, and Jean-François Flot for the useful discussions and/or assistance with figures. We thank Prof. Zeng Xiaoqi from the Ocean University of China for sharing the samples collected from Paracel Islands, South China Sea. Financial support was provided by the Australian Research Council (DE130100688 to ASH) and KAUST baseline research funds (to MLB).

Funding Information:
Acknowledgements This study was conducted under Great Barrier Reef Marine Park Authority permit G11/34319.1 and QLD Fisheries permit 103256. Ethical approval was obtained from James Cook University (permit A1267). We thank the Lizard Island Research Station staff for fieldwork support and the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory for laboratory support. We thank Gerry Allen, Tane Sinclair-Taylor, and Jean-François Flot for the useful discussions and/or assistance with figures. We thank Prof. Zeng Xiaoqi from the Ocean University of China for sharing the samples collected from Paracel Islands, South China Sea. Financial support was provided by the Australian Research Council (DE130100688 to ASH) and KAUST baseline research funds (to MLB).

Publisher Copyright:
© 2017, Senckenberg Gesellschaft für Naturforschung and Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Damselfish
Molecular identification
Natural hybridization

ASJC Scopus subject areas

Oceanography
Ecology, Evolution, Behavior and Systematics
Aquatic Science

Access to Document

10.1007/s12526-017-0819-8

Cite this

@article{5b49a1dedacc42de9a849502b9bb336f,

title = "Molecular confirmation of hybridization between Dascyllus reticulatus × Dascyllus aruanus from the Great Barrier Reef",

abstract = "To date, more than 81 species of tropical coral reef fish have been reported to hybridize in nature, spanning multiple families, including the Chaetodontidae, Pomacanthidae, and Labridae. Hybridization, however, is seemingly rare among benthic nesting species that engage in pair spawning, such as the Pomacentridae. Here, we present evidence for the first molecularly confirmed record of hybridization within the genus Dascyllus; D. aruanus and D. reticulatus. Interestingly, although many hybridization events are attributed to peripheral range effects or areas of limited overlap among otherwise allopatric species, this hybrid individual was collected from the northern Great Barrier Reef, centrally located within the distribution ranges of both species. The hybrid exhibited coloration and meristic counts intermediate between D. aruanus and D. reticulatus. Diagnostic genetic markers and subsequent microsatellites analysis confirmed that this individual was a hybrid offspring of D. aruanus and D. reticulatus, with the latter providing the maternal contribution. The occurrence of the D. aruanus × D. reticulatus hybrid on the Great Barrier Reef represents an exception to the otherwise species-specific haplotypes. The nuclear diagnostic marker which was identified during this study could serve as a hybrid indicator and benefit future hybrid investigations for hybridization between these two species.",

keywords = "Damselfish, Molecular identification, Natural hybridization",

author = "Song He and Johansen, {Jacob L.} and Hoey, {Andrew S.} and Pappas, {Melissa K.} and Berumen, {Michael L.}",

note = "Funding Information: This study was conducted under Great Barrier Reef Marine Park Authority permit G11/34319.1 and QLD Fisheries permit 103256. Ethical approval was obtained from James Cook University (permit A1267). We thank the Lizard Island Research Station staff for fieldwork support and the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory for laboratory support. We thank Gerry Allen, Tane Sinclair-Taylor, and Jean-Fran{\c c}ois Flot for the useful discussions and/or assistance with figures. We thank Prof. Zeng Xiaoqi from the Ocean University of China for sharing the samples collected from Paracel Islands, South China Sea. Financial support was provided by the Australian Research Council (DE130100688 to ASH) and KAUST baseline research funds (to MLB). Funding Information: Acknowledgements This study was conducted under Great Barrier Reef Marine Park Authority permit G11/34319.1 and QLD Fisheries permit 103256. Ethical approval was obtained from James Cook University (permit A1267). We thank the Lizard Island Research Station staff for fieldwork support and the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory for laboratory support. We thank Gerry Allen, Tane Sinclair-Taylor, and Jean-Fran{\c c}ois Flot for the useful discussions and/or assistance with figures. We thank Prof. Zeng Xiaoqi from the Ocean University of China for sharing the samples collected from Paracel Islands, South China Sea. Financial support was provided by the Australian Research Council (DE130100688 to ASH) and KAUST baseline research funds (to MLB). Publisher Copyright: {\textcopyright} 2017, Senckenberg Gesellschaft f{\"u}r Naturforschung and Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2019",

month = feb,

day = "5",

doi = "10.1007/s12526-017-0819-8",

language = "English (US)",

volume = "49",

pages = "395--404",

journal = "Marine Biodiversity",

issn = "1867-1616",

publisher = "Springer Verlag",

number = "1",

}

TY - JOUR

T1 - Molecular confirmation of hybridization between Dascyllus reticulatus × Dascyllus aruanus from the Great Barrier Reef

AU - He, Song

AU - Johansen, Jacob L.

AU - Hoey, Andrew S.

AU - Pappas, Melissa K.

AU - Berumen, Michael L.

N1 - Funding Information: This study was conducted under Great Barrier Reef Marine Park Authority permit G11/34319.1 and QLD Fisheries permit 103256. Ethical approval was obtained from James Cook University (permit A1267). We thank the Lizard Island Research Station staff for fieldwork support and the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory for laboratory support. We thank Gerry Allen, Tane Sinclair-Taylor, and Jean-François Flot for the useful discussions and/or assistance with figures. We thank Prof. Zeng Xiaoqi from the Ocean University of China for sharing the samples collected from Paracel Islands, South China Sea. Financial support was provided by the Australian Research Council (DE130100688 to ASH) and KAUST baseline research funds (to MLB). Funding Information: Acknowledgements This study was conducted under Great Barrier Reef Marine Park Authority permit G11/34319.1 and QLD Fisheries permit 103256. Ethical approval was obtained from James Cook University (permit A1267). We thank the Lizard Island Research Station staff for fieldwork support and the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory for laboratory support. We thank Gerry Allen, Tane Sinclair-Taylor, and Jean-François Flot for the useful discussions and/or assistance with figures. We thank Prof. Zeng Xiaoqi from the Ocean University of China for sharing the samples collected from Paracel Islands, South China Sea. Financial support was provided by the Australian Research Council (DE130100688 to ASH) and KAUST baseline research funds (to MLB). Publisher Copyright: © 2017, Senckenberg Gesellschaft für Naturforschung and Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2019/2/5

Y1 - 2019/2/5

N2 - To date, more than 81 species of tropical coral reef fish have been reported to hybridize in nature, spanning multiple families, including the Chaetodontidae, Pomacanthidae, and Labridae. Hybridization, however, is seemingly rare among benthic nesting species that engage in pair spawning, such as the Pomacentridae. Here, we present evidence for the first molecularly confirmed record of hybridization within the genus Dascyllus; D. aruanus and D. reticulatus. Interestingly, although many hybridization events are attributed to peripheral range effects or areas of limited overlap among otherwise allopatric species, this hybrid individual was collected from the northern Great Barrier Reef, centrally located within the distribution ranges of both species. The hybrid exhibited coloration and meristic counts intermediate between D. aruanus and D. reticulatus. Diagnostic genetic markers and subsequent microsatellites analysis confirmed that this individual was a hybrid offspring of D. aruanus and D. reticulatus, with the latter providing the maternal contribution. The occurrence of the D. aruanus × D. reticulatus hybrid on the Great Barrier Reef represents an exception to the otherwise species-specific haplotypes. The nuclear diagnostic marker which was identified during this study could serve as a hybrid indicator and benefit future hybrid investigations for hybridization between these two species.

AB - To date, more than 81 species of tropical coral reef fish have been reported to hybridize in nature, spanning multiple families, including the Chaetodontidae, Pomacanthidae, and Labridae. Hybridization, however, is seemingly rare among benthic nesting species that engage in pair spawning, such as the Pomacentridae. Here, we present evidence for the first molecularly confirmed record of hybridization within the genus Dascyllus; D. aruanus and D. reticulatus. Interestingly, although many hybridization events are attributed to peripheral range effects or areas of limited overlap among otherwise allopatric species, this hybrid individual was collected from the northern Great Barrier Reef, centrally located within the distribution ranges of both species. The hybrid exhibited coloration and meristic counts intermediate between D. aruanus and D. reticulatus. Diagnostic genetic markers and subsequent microsatellites analysis confirmed that this individual was a hybrid offspring of D. aruanus and D. reticulatus, with the latter providing the maternal contribution. The occurrence of the D. aruanus × D. reticulatus hybrid on the Great Barrier Reef represents an exception to the otherwise species-specific haplotypes. The nuclear diagnostic marker which was identified during this study could serve as a hybrid indicator and benefit future hybrid investigations for hybridization between these two species.

KW - Damselfish

KW - Molecular identification

KW - Natural hybridization

UR - http://www.scopus.com/inward/record.url?scp=85034248758&partnerID=8YFLogxK

U2 - 10.1007/s12526-017-0819-8

DO - 10.1007/s12526-017-0819-8

M3 - Article

AN - SCOPUS:85034248758

SN - 1867-1616

VL - 49

SP - 395

EP - 404

JO - Marine Biodiversity

JF - Marine Biodiversity

IS - 1

ER -

Molecular confirmation of hybridization between Dascyllus reticulatus × Dascyllus aruanus from the Great Barrier Reef

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this