Molecular biology: Antisense transcription in the mammalian transcriptome

S. Katayama; Y. Tomaru; T. Kasukawa; K. Waki; M. Nakanishi; M. Nakamura; H. Nishida; C. C. Yap; M. Suzuki; J. Kawai; H. Suzuki; P. Carninci; Y. Hayashizaki; C. Wells; M. Frith; T. Ravasi; K. C. Pang; J. Hallinan; J. Mattick; D. A. Hume; L. Lipovich; S. Batalov; P. G. Engström; Y. Mizuno; M. A. Faghihi; A. Sandelin; A. M. Chalk; S. Mottagui-Tabar; Z. Liang; B. Lenhard; C. Wahlestedt

doi:10.1126/science.1112009

Molecular biology: Antisense transcription in the mammalian transcriptome

S. Katayama, Y. Tomaru, T. Kasukawa, K. Waki, M. Nakanishi, M. Nakamura, H. Nishida, C. C. Yap, M. Suzuki, J. Kawai, H. Suzuki, P. Carninci^*, Y. Hayashizaki, C. Wells, M. Frith, T. Ravasi, K. C. Pang, J. Hallinan, J. Mattick, D. A. HumeL. Lipovich, S. Batalov, P. G. Engström, Y. Mizuno, M. A. Faghihi, A. Sandelin, A. M. Chalk, S. Mottagui-Tabar, Z. Liang, B. Lenhard, C. Wahlestedt

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of the corresponding sense transcripts (RNA interference), as well as gene silencing at the chromatin level. Global transcriptome analysis provides evidence that a large proportion of the genome can produce transcripts from both strands, and that antisense transcripts commonly link neighboring "genes" in complex loci into chains of linked transcriptional units. Expression profiling reveals frequent concordant regulation of sense/antisense pairs. We present experimental evidence that perturbation of an antisense RNA can alter the expression of sense messenger RNAs, suggesting that antisense transcription contributes to control of transcriptional outputs in mammals.

Original language	English (US)
Pages (from-to)	1564-1566
Number of pages	3
Journal	SCIENCE
Volume	309
Issue number	5740
DOIs	https://doi.org/10.1126/science.1112009
State	Published - Sep 2 2005
Externally published	Yes

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Katayama, S., Tomaru, Y., Kasukawa, T., Waki, K., Nakanishi, M., Nakamura, M., Nishida, H., Yap, C. C., Suzuki, M., Kawai, J., Suzuki, H., Carninci, P., Hayashizaki, Y., Wells, C., Frith, M., Ravasi, T., Pang, K. C., Hallinan, J., Mattick, J., ... Wahlestedt, C. (2005). Molecular biology: Antisense transcription in the mammalian transcriptome. SCIENCE, 309(5740), 1564-1566. https://doi.org/10.1126/science.1112009

@article{668d7307d33340e89059923c38aa05fc,

title = "Molecular biology: Antisense transcription in the mammalian transcriptome",

abstract = "Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of the corresponding sense transcripts (RNA interference), as well as gene silencing at the chromatin level. Global transcriptome analysis provides evidence that a large proportion of the genome can produce transcripts from both strands, and that antisense transcripts commonly link neighboring {"}genes{"} in complex loci into chains of linked transcriptional units. Expression profiling reveals frequent concordant regulation of sense/antisense pairs. We present experimental evidence that perturbation of an antisense RNA can alter the expression of sense messenger RNAs, suggesting that antisense transcription contributes to control of transcriptional outputs in mammals.",

author = "S. Katayama and Y. Tomaru and T. Kasukawa and K. Waki and M. Nakanishi and M. Nakamura and H. Nishida and Yap, {C. C.} and M. Suzuki and J. Kawai and H. Suzuki and P. Carninci and Y. Hayashizaki and C. Wells and M. Frith and T. Ravasi and Pang, {K. C.} and J. Hallinan and J. Mattick and Hume, {D. A.} and L. Lipovich and S. Batalov and Engstr{\"o}m, {P. G.} and Y. Mizuno and Faghihi, {M. A.} and A. Sandelin and Chalk, {A. M.} and S. Mottagui-Tabar and Z. Liang and B. Lenhard and C. Wahlestedt",

year = "2005",

month = sep,

day = "2",

doi = "10.1126/science.1112009",

language = "English (US)",

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Katayama, S, Tomaru, Y, Kasukawa, T, Waki, K, Nakanishi, M, Nakamura, M, Nishida, H, Yap, CC, Suzuki, M, Kawai, J, Suzuki, H, Carninci, P, Hayashizaki, Y, Wells, C, Frith, M, Ravasi, T, Pang, KC, Hallinan, J, Mattick, J, Hume, DA, Lipovich, L, Batalov, S, Engström, PG, Mizuno, Y, Faghihi, MA, Sandelin, A, Chalk, AM, Mottagui-Tabar, S, Liang, Z, Lenhard, B & Wahlestedt, C 2005, 'Molecular biology: Antisense transcription in the mammalian transcriptome', SCIENCE, vol. 309, no. 5740, pp. 1564-1566. https://doi.org/10.1126/science.1112009

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T1 - Molecular biology

T2 - Antisense transcription in the mammalian transcriptome

AU - Katayama, S.

AU - Tomaru, Y.

AU - Kasukawa, T.

AU - Waki, K.

AU - Nakanishi, M.

AU - Nakamura, M.

AU - Nishida, H.

AU - Yap, C. C.

AU - Suzuki, M.

AU - Kawai, J.

AU - Suzuki, H.

AU - Carninci, P.

AU - Hayashizaki, Y.

AU - Wells, C.

AU - Frith, M.

AU - Ravasi, T.

AU - Pang, K. C.

AU - Hallinan, J.

AU - Mattick, J.

AU - Hume, D. A.

AU - Lipovich, L.

AU - Batalov, S.

AU - Engström, P. G.

AU - Mizuno, Y.

AU - Faghihi, M. A.

AU - Sandelin, A.

AU - Chalk, A. M.

AU - Mottagui-Tabar, S.

AU - Liang, Z.

AU - Lenhard, B.

AU - Wahlestedt, C.

PY - 2005/9/2

Y1 - 2005/9/2

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AB - Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of the corresponding sense transcripts (RNA interference), as well as gene silencing at the chromatin level. Global transcriptome analysis provides evidence that a large proportion of the genome can produce transcripts from both strands, and that antisense transcripts commonly link neighboring "genes" in complex loci into chains of linked transcriptional units. Expression profiling reveals frequent concordant regulation of sense/antisense pairs. We present experimental evidence that perturbation of an antisense RNA can alter the expression of sense messenger RNAs, suggesting that antisense transcription contributes to control of transcriptional outputs in mammals.

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DO - 10.1126/science.1112009

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AN - SCOPUS:24644472515

SN - 0036-8075

VL - 309

SP - 1564

EP - 1566

JO - SCIENCE

JF - SCIENCE

IS - 5740

ER -

Molecular biology: Antisense transcription in the mammalian transcriptome

Abstract

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