Abstract
Gradient elution separations of polystyrene standards in a monolithic molded 50 x 8 mm I.D. poly(styrene-co-divinylbenzene) rod column and in 50 x 8 mm I.D. and 30 x 4.1 mm I.D. columns packed with porous and non-porous poly(styrene-co-divinylbenzene) beads has been carried out. All of these separation media differ in shape and porosity. Excellent separations of eight polystyrene standards were achieved with both the molded monolithic rod and porous beads at moderate flow-rates. However, the monolithic medium proved to be superior for high-speed separations using very steep gradients at a flow-rate of 20 ml/min. Three polystyrene standards were separated in the rod column within 4 s. The separation in the column packed with non-porous beads was poor at flow-rates of 2-8 ml/min, while higher flow-rates led to an unacceptably high back pressure.
Original language | English (US) |
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Pages (from-to) | 59-66 |
Number of pages | 8 |
Journal | Journal of Chromatography A |
Volume | 752 |
Issue number | 1-2 |
DOIs | |
State | Published - Nov 1 1996 |
Externally published | Yes |
Bibliographical note
Funding Information:Support of this research by a grant of the National Institutes of Health (GM-48364) and by a gift from Exxon Chemical Corporation is gratefully acknowledged. Thanks are also due to Comenius University, Bratislava, Slovak Republic for granting a leave of absence to M.P.
Keywords
- Monolithic column
- Poly(styrene-co-divinylbenzene)
- Polymers
- Polystyrenes
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry