Modified poly(glycidyl metharylate-co-ethylene dimethacrylate) continuous rod columns for preparative-scale ion-exchange chromatography of proteins

Frantisek Svec; Jean M.J. Fréchet

doi:10.1016/0021-9673(94)01021-6

Modified poly(glycidyl metharylate-co-ethylene dimethacrylate) continuous rod columns for preparative-scale ion-exchange chromatography of proteins

Frantisek Svec^*, Jean M.J. Fréchet

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

193 Scopus citations

Abstract

A continuous rod of porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) has been prepared by a free radical polymerization within the confines of a 300 × 8 mm I.D. chromatographic column. The epoxide groups of the rod have been modified by a reaction with diethylamine that affords ionizable functionalities required for the ion-exchange chromatographic mode. The properties of this rod column have been characterized and the column has been used successfully for the chromatographic separation of proteins. The column exhibits a dynamic capacity that exceeds 300 mg at a flow velocity of 200 cm/min. An excellent selectivity allows the separation of up to 300 mg of a protein mixture in a single run.

Original language	English (US)
Pages (from-to)	89-95
Number of pages	7
Journal	Journal of Chromatography A
Volume	702
Issue number	1-2
DOIs	https://doi.org/10.1016/0021-9673(94)01021-6
State	Published - May 19 1995
Externally published	Yes

Bibliographical note

Funding Information:
Support of this research by a grant of the National Institute of Health (GM 48364-02) is gratefully acknowledged. This work also made use of MRL Central Facilities supported by the National Science Foundation under Award No. DMR-9121654.

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

Access to Document

10.1016/0021-9673(94)01021-6

Cite this

@article{c9437e91425e4160b8953256191e8bbd,

title = "Modified poly(glycidyl metharylate-co-ethylene dimethacrylate) continuous rod columns for preparative-scale ion-exchange chromatography of proteins",

abstract = "A continuous rod of porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) has been prepared by a free radical polymerization within the confines of a 300 × 8 mm I.D. chromatographic column. The epoxide groups of the rod have been modified by a reaction with diethylamine that affords ionizable functionalities required for the ion-exchange chromatographic mode. The properties of this rod column have been characterized and the column has been used successfully for the chromatographic separation of proteins. The column exhibits a dynamic capacity that exceeds 300 mg at a flow velocity of 200 cm/min. An excellent selectivity allows the separation of up to 300 mg of a protein mixture in a single run.",

author = "Frantisek Svec and Fr{\'e}chet, {Jean M.J.}",

note = "Funding Information: Support of this research by a grant of the National Institute of Health (GM 48364-02) is gratefully acknowledged. This work also made use of MRL Central Facilities supported by the National Science Foundation under Award No. DMR-9121654.",

year = "1995",

month = may,

day = "19",

doi = "10.1016/0021-9673(94)01021-6",

language = "English (US)",

volume = "702",

pages = "89--95",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Modified poly(glycidyl metharylate-co-ethylene dimethacrylate) continuous rod columns for preparative-scale ion-exchange chromatography of proteins

AU - Svec, Frantisek

AU - Fréchet, Jean M.J.

N1 - Funding Information: Support of this research by a grant of the National Institute of Health (GM 48364-02) is gratefully acknowledged. This work also made use of MRL Central Facilities supported by the National Science Foundation under Award No. DMR-9121654.

PY - 1995/5/19

Y1 - 1995/5/19

N2 - A continuous rod of porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) has been prepared by a free radical polymerization within the confines of a 300 × 8 mm I.D. chromatographic column. The epoxide groups of the rod have been modified by a reaction with diethylamine that affords ionizable functionalities required for the ion-exchange chromatographic mode. The properties of this rod column have been characterized and the column has been used successfully for the chromatographic separation of proteins. The column exhibits a dynamic capacity that exceeds 300 mg at a flow velocity of 200 cm/min. An excellent selectivity allows the separation of up to 300 mg of a protein mixture in a single run.

AB - A continuous rod of porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) has been prepared by a free radical polymerization within the confines of a 300 × 8 mm I.D. chromatographic column. The epoxide groups of the rod have been modified by a reaction with diethylamine that affords ionizable functionalities required for the ion-exchange chromatographic mode. The properties of this rod column have been characterized and the column has been used successfully for the chromatographic separation of proteins. The column exhibits a dynamic capacity that exceeds 300 mg at a flow velocity of 200 cm/min. An excellent selectivity allows the separation of up to 300 mg of a protein mixture in a single run.

UR - http://www.scopus.com/inward/record.url?scp=0029077511&partnerID=8YFLogxK

U2 - 10.1016/0021-9673(94)01021-6

DO - 10.1016/0021-9673(94)01021-6

M3 - Article

C2 - 7599741

AN - SCOPUS:0029077511

SN - 0021-9673

VL - 702

SP - 89

EP - 95

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Modified poly(glycidyl metharylate-co-ethylene dimethacrylate) continuous rod columns for preparative-scale ion-exchange chromatography of proteins

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this