TY - JOUR
T1 - mKikGR, a monomeric photoswitchable fluorescent protein
AU - Habuchi, Satoshi
AU - Tsutsui, Hidekazu
AU - Kochaniak, Anna B.
AU - Miyawaki, Atsushi
AU - van Oijen, Antoine M.
PY - 2008/12/15
Y1 - 2008/12/15
N2 - The recent demonstration and utilization of fluorescent proteins whose fluorescence can be switched on and off has greatly expanded the toolkit of molecular and cell biology. These photoswitchable proteins have facilitated the characterization of specifically tagged molecular species in the cell and have enabled fluorescence imaging of intracellular structures with a resolution far below the classical diffraction limit of light. Applications are limited, however, by the fast photobleaching, slow photoswitching, and oligomerization typical for photoswitchable proteins currently available. Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates. Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging.
AB - The recent demonstration and utilization of fluorescent proteins whose fluorescence can be switched on and off has greatly expanded the toolkit of molecular and cell biology. These photoswitchable proteins have facilitated the characterization of specifically tagged molecular species in the cell and have enabled fluorescence imaging of intracellular structures with a resolution far below the classical diffraction limit of light. Applications are limited, however, by the fast photobleaching, slow photoswitching, and oligomerization typical for photoswitchable proteins currently available. Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates. Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging.
UR - http://www.scopus.com/inward/record.url?scp=58049208311&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0003944
DO - 10.1371/journal.pone.0003944
M3 - Article
C2 - 19079591
AN - SCOPUS:58049208311
SN - 1932-6203
VL - 3
JO - PloS one
JF - PloS one
IS - 12
M1 - e3944
ER -