mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells

Daming Zhang; Guang Yang; Xin Chen; Chunmei Li; Lu Wang; Yaohua Liu; Dayong Han; Huailei Liu; Xu Hou; Weiguang Zhang; Chenguang Li; Zhanqiang Han; Xin Gao; Shiguang Zhao

doi:10.1007/s12031-014-0230-x

mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells

Daming Zhang, Guang Yang, Xin Chen, Chunmei Li, Lu Wang, Yaohua Liu, Dayong Han, Huailei Liu, Xu Hou, Weiguang Zhang, Chenguang Li, Zhanqiang Han, Xin Gao, Shiguang Zhao

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.

Original language	English (US)
Pages (from-to)	637-644
Number of pages	8
Journal	Journal of Molecular Neuroscience
Volume	53
Issue number	4
DOIs	https://doi.org/10.1007/s12031-014-0230-x
State	Published - Jan 28 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12031-014-0230-x

Cite this

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title = "mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells",

abstract = "MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. {\textcopyright} 2014 Springer Science+Business Media.",

author = "Daming Zhang and Guang Yang and Xin Chen and Chunmei Li and Lu Wang and Yaohua Liu and Dayong Han and Huailei Liu and Xu Hou and Weiguang Zhang and Chenguang Li and Zhanqiang Han and Xin Gao and Shiguang Zhao",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).",

year = "2014",

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doi = "10.1007/s12031-014-0230-x",

language = "English (US)",

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T1 - mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells

AU - Zhang, Daming

AU - Yang, Guang

AU - Chen, Xin

AU - Li, Chunmei

AU - Wang, Lu

AU - Liu, Yaohua

AU - Han, Dayong

AU - Liu, Huailei

AU - Hou, Xu

AU - Zhang, Weiguang

AU - Li, Chenguang

AU - Han, Zhanqiang

AU - Gao, Xin

AU - Zhao, Shiguang

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).

PY - 2014/1/28

Y1 - 2014/1/28

N2 - MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.

AB - MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.

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SP - 637

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JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

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ER -

mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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