TY - JOUR
T1 - Mining the deep Red-Sea brine pool microbial community for anticancer therapeutics.
AU - Esau, Luke
AU - Zhang, Guishan
AU - Sagar, Sunil
AU - Stingl, Ulrich
AU - Bajic, Vladimir B.
AU - Kaur, Mandeep
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: Authors want to thank Dr. Andre Antunes and Dr. Tyas Hikmawan for collecting samples.
PY - 2019/6/20
Y1 - 2019/6/20
N2 - BACKGROUND:
Microbial species in the brine pools of the Red Sea and the brine pool-seawater interfaces are exposed to high temperature, high salinity, low oxygen levels and high concentrations of heavy metals. As adaptations to these harsh conditions require a large suite of secondary metabolites, these microbes have a huge potential as a source of novel anticancer molecules. METHODS:A total of 60 ethyl-acetate extracts of newly isolated strains from extreme environments of the Red-Sea were isolated and tested against several human cancer cell lines for potential cytotoxic and apoptotic activities.
RESULTS:
Isolates from the Erba brine-pool accounted for 50% of active bacterial extracts capable of inducing 30% or greater inhibition of cell growth. Among the 60 extracts screened, seven showed selectivity towards triple negative BT20 cells compared to normal fibroblasts.
CONCLUSION:
In this study, we identified several extracts able to induce caspase-dependent apoptosis in various cancer cell lines. Further investigations and isolation of the active compounds of these Red Sea brine pool microbes may offer a chemotherapeutic potential for cancers with limited treatment options.
AB - BACKGROUND:
Microbial species in the brine pools of the Red Sea and the brine pool-seawater interfaces are exposed to high temperature, high salinity, low oxygen levels and high concentrations of heavy metals. As adaptations to these harsh conditions require a large suite of secondary metabolites, these microbes have a huge potential as a source of novel anticancer molecules. METHODS:A total of 60 ethyl-acetate extracts of newly isolated strains from extreme environments of the Red-Sea were isolated and tested against several human cancer cell lines for potential cytotoxic and apoptotic activities.
RESULTS:
Isolates from the Erba brine-pool accounted for 50% of active bacterial extracts capable of inducing 30% or greater inhibition of cell growth. Among the 60 extracts screened, seven showed selectivity towards triple negative BT20 cells compared to normal fibroblasts.
CONCLUSION:
In this study, we identified several extracts able to induce caspase-dependent apoptosis in various cancer cell lines. Further investigations and isolation of the active compounds of these Red Sea brine pool microbes may offer a chemotherapeutic potential for cancers with limited treatment options.
UR - http://hdl.handle.net/10754/656338
UR - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-019-2554-0
UR - http://www.scopus.com/inward/record.url?scp=85067566512&partnerID=8YFLogxK
U2 - 10.1186/s12906-019-2554-0
DO - 10.1186/s12906-019-2554-0
M3 - Article
C2 - 31221160
SN - 1472-6882
VL - 19
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
IS - 1
ER -
