Minimally-invasive, real-time, non-destructive, species-independent phytohormone biosensor for precision farming

Abdullah Bukhamsin*, Abdellatif Ait Lahcen, Jose De Oliveira Filho, Saptami Shetty, Ikram Blilou, Jürgen Kosel, Khaled Nabil Salama

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

To keep up with population growth, precision farming technologies must be implemented to sustainably increase agricultural output. The impact of such technologies can be expanded by monitoring phytohormones, such as salicylic acid. In this study, we present a plant-wearable electrochemical sensor for in situ detection of salicylic acid. The sensor utilizes microneedle-based electrodes that are functionalized with a layer of salicylic acid selective magnetic molecularly imprinted polymers. The sensor's capability to detect the phytohormone is demonstrated both in vitro and in vivo with a limit of detection of 2.74 μM and a range of detection that can reach as high as 150 μM. Furthermore, the selectivity of the sensor is verified by testing the sensor on commonly occurring phytohormones. Finally, we demonstrate the capability of the sensor to detect the onset of fungal infestation in Tobacco 5 min post-inoculation. This work shows that the sensor could serve as a promising platform for continuous and non-destructive monitoring in the field and as a fundamental research tool when coupled with a portable potentiostat.

Original languageEnglish (US)
Article number114515
JournalBiosensors and Bioelectronics
Volume214
DOIs
StatePublished - Oct 15 2022

Bibliographical note

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

  • Lightweight custom potentiostat
  • Minimally invasive monitoring
  • Molecularly imprinted polymers
  • Precision farming
  • Salicylic acid

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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