Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead

Kazuo Yamauchi; Shizuo Yamasaki; Rui Takahashi; Tetsuo Asakura

doi:10.1016/j.ssnmr.2010.06.002

Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead

Kazuo Yamauchi, Shizuo Yamasaki, Rui Takahashi, Tetsuo Asakura

Imaging & Characterization Core Lab

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Conformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. © 2010 Elsevier Inc. All rights reserved.

Original language	English (US)
Pages (from-to)	27-30
Number of pages	4
Journal	Solid State Nuclear Magnetic Resonance
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/j.ssnmr.2010.06.002
State	Published - Jul 2010

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors acknowledge the financial support of SENTAN, JST for the NMR probehead developments. TA acknowledges support from a Grant-in-Aid for Scientific Research from Ministry of Education, Science, Culture and Supports of Japan (18105007) and also the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan. The authors acknowledge Prof. Mike Williamson, University of Sheffield, UK for many suggestions and careful reading of our paper.

ASJC Scopus subject areas

Instrumentation
General Chemistry
Nuclear and High Energy Physics
Radiation

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title = "Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead",

abstract = "Conformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. {\textcopyright} 2010 Elsevier Inc. All rights reserved.",

author = "Kazuo Yamauchi and Shizuo Yamasaki and Rui Takahashi and Tetsuo Asakura",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors acknowledge the financial support of SENTAN, JST for the NMR probehead developments. TA acknowledges support from a Grant-in-Aid for Scientific Research from Ministry of Education, Science, Culture and Supports of Japan (18105007) and also the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan. The authors acknowledge Prof. Mike Williamson, University of Sheffield, UK for many suggestions and careful reading of our paper.",

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AU - Takahashi, Rui

AU - Asakura, Tetsuo

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors acknowledge the financial support of SENTAN, JST for the NMR probehead developments. TA acknowledges support from a Grant-in-Aid for Scientific Research from Ministry of Education, Science, Culture and Supports of Japan (18105007) and also the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan. The authors acknowledge Prof. Mike Williamson, University of Sheffield, UK for many suggestions and careful reading of our paper.

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N2 - Conformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. © 2010 Elsevier Inc. All rights reserved.

AB - Conformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. © 2010 Elsevier Inc. All rights reserved.

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Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead

Abstract

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