Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci

Hugo B. Harrison; Pablo Saenz Agudelo; Manalle Al-Salamah; Vanessa Messmer; Morgan S. Pratchett; Michael L. Berumen

doi:10.1007/s12686-015-0462-2

Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci

Hugo B. Harrison, Pablo Saenz Agudelo, Manalle Al-Salamah, Vanessa Messmer, Morgan S. Pratchett, Michael L. Berumen

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Population outbreaks of crown-of-thorns starfish (Acanthaster spp.) represent one of the most significant biological disturbances on Indo-Pacific coral reefs. Here, we combine 15 published and 11 newly isolated polymorphic microsatellite markers from the coral-eating starfish, A. cf. planci and describe their integration into four multiplex PCRs. All markers were polymorphic with a mean of 11.7 ± 1.9 SE alleles per locus and an average observed heterozygosity of 0.619 ± 0.049 SE across 195 genotyped individuals from the Great Barrier Reef. This multiplex assay provides an effective means of investigating the population dynamics of crown-of-thorns starfish and the initiation and spread of population outbreaks.

Original language	English (US)
Pages (from-to)	627-630
Number of pages	4
Journal	Conservation Genetics Resources
Volume	7
Issue number	3
DOIs	https://doi.org/10.1007/s12686-015-0462-2
State	Published - Mar 20 2015

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10.1007/s12686-015-0462-2

https://repository.kaust.edu.sa/bitstream/10754/567096/1/COTs%20Microsatellite%20ConsGenetRes_Final.doc

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title = "Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci",

abstract = "Population outbreaks of crown-of-thorns starfish (Acanthaster spp.) represent one of the most significant biological disturbances on Indo-Pacific coral reefs. Here, we combine 15 published and 11 newly isolated polymorphic microsatellite markers from the coral-eating starfish, A. cf. planci and describe their integration into four multiplex PCRs. All markers were polymorphic with a mean of 11.7 ± 1.9 SE alleles per locus and an average observed heterozygosity of 0.619 ± 0.049 SE across 195 genotyped individuals from the Great Barrier Reef. This multiplex assay provides an effective means of investigating the population dynamics of crown-of-thorns starfish and the initiation and spread of population outbreaks.",

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T1 - Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci

AU - Harrison, Hugo B.

AU - Saenz Agudelo, Pablo

AU - Al-Salamah, Manalle

AU - Messmer, Vanessa

AU - Pratchett, Morgan S.

AU - Berumen, Michael L.

N1 - KAUST Repository Item: Exported on 2020-10-01

PY - 2015/3/20

Y1 - 2015/3/20

N2 - Population outbreaks of crown-of-thorns starfish (Acanthaster spp.) represent one of the most significant biological disturbances on Indo-Pacific coral reefs. Here, we combine 15 published and 11 newly isolated polymorphic microsatellite markers from the coral-eating starfish, A. cf. planci and describe their integration into four multiplex PCRs. All markers were polymorphic with a mean of 11.7 ± 1.9 SE alleles per locus and an average observed heterozygosity of 0.619 ± 0.049 SE across 195 genotyped individuals from the Great Barrier Reef. This multiplex assay provides an effective means of investigating the population dynamics of crown-of-thorns starfish and the initiation and spread of population outbreaks.

AB - Population outbreaks of crown-of-thorns starfish (Acanthaster spp.) represent one of the most significant biological disturbances on Indo-Pacific coral reefs. Here, we combine 15 published and 11 newly isolated polymorphic microsatellite markers from the coral-eating starfish, A. cf. planci and describe their integration into four multiplex PCRs. All markers were polymorphic with a mean of 11.7 ± 1.9 SE alleles per locus and an average observed heterozygosity of 0.619 ± 0.049 SE across 195 genotyped individuals from the Great Barrier Reef. This multiplex assay provides an effective means of investigating the population dynamics of crown-of-thorns starfish and the initiation and spread of population outbreaks.

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UR - http://link.springer.com/10.1007/s12686-015-0462-2

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DO - 10.1007/s12686-015-0462-2

M3 - Article

SN - 1877-7252

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JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 3

ER -

Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci

Abstract

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