Microfluidics & nanotechnology: Towards fully integrated analytical devices for the detection of cancer biomarkers

Gerardo Perozziello; Patrizio Candeloro; Francesco T. Gentile; Annalisa Nicastri; Angela Mena Perri; Maria Laura Coluccio; A. Adamo; Francesca Pardeo; Rossella Catalano; Elvira Parrotta; Horacio Dante Espinosa; Giovanni Cuda; Enzo M. Di Fabrizio

doi:10.1039/c4ra10486b

Microfluidics & nanotechnology: Towards fully integrated analytical devices for the detection of cancer biomarkers

Gerardo Perozziello, Patrizio Candeloro, Francesco T. Gentile, Annalisa Nicastri, Angela Mena Perri, Maria Laura Coluccio, A. Adamo, Francesca Pardeo, Rossella Catalano, Elvira Parrotta, Horacio Dante Espinosa, Giovanni Cuda, Enzo M. Di Fabrizio

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

In this paper, we describe an innovative modular microfluidic platform allowing filtering, concentration and analysis of peptides from a complex mixture. The platform is composed of a microfluidic filtering device and a superhydrophobic surface integrating surface enhanced Raman scattering (SERS) sensors. The microfluidic device was used to filter specific peptides (MW 1553.73 D) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancers, from albumin (66.5 KD), the most represented protein in human plasma. The filtering process consisted of driving the complex mixture through a porous membrane having a cut-off of 12-14 kD by hydrodynamic flow. The filtered samples coming out of the microfluidic device were subsequently deposited on a superhydrophobic surface formed by micro pillars on top of which nanograins were fabricated. The nanograins coupled to a Raman spectroscopy instrument acted as a SERS sensor and allowed analysis of the filtered sample on top of the surface once it evaporated. By using the presented platform, we demonstrate being able to sort small peptides from bigger proteins and to detect them by using a label-free technique at a resolution down to 0.1 ng μL-1. The combination of microfluidics and nanotechnology to develop the presented microfluidic platform may give rise to a new generation of biosensors capable of detecting low concentration samples from complex mixtures without the need for any sample pretreatment or labelling. The developed devices could have future applications in the field of early diagnosis of severe illnesses, e.g. early cancer detection. This journal is

Original language	English (US)
Pages (from-to)	55590-55598
Number of pages	9
Journal	RSC Adv.
Volume	4
Issue number	98
DOIs	https://doi.org/10.1039/c4ra10486b
State	Published - 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338) the projects PON01_02834 "Prometeo", PONa3_00435 "Biomedpark@UMG" and FIRB "ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUPB41J09000110005) financed from the Ministry of Education and Research, the project for Young researchers financed from the Ministry of Health "High Troughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) and Cancer biomarker detection using micro-structured/superhydrophobic surfaces and advanced spectroscopy techniques (CUP J65C13001370001, project no. GR-2010-2320665) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.

ASJC Scopus subject areas

General Chemical Engineering
General Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/c4ra10486b

Cite this

Perozziello, G., Candeloro, P., Gentile, F. T., Nicastri, A., Perri, A. M., Coluccio, M. L., Adamo, A., Pardeo, F., Catalano, R., Parrotta, E., Espinosa, H. D., Cuda, G., & Di Fabrizio, E. M. (2014). Microfluidics & nanotechnology: Towards fully integrated analytical devices for the detection of cancer biomarkers. RSC Adv., 4(98), 55590-55598. https://doi.org/10.1039/c4ra10486b

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title = "Microfluidics & nanotechnology: Towards fully integrated analytical devices for the detection of cancer biomarkers",

abstract = "In this paper, we describe an innovative modular microfluidic platform allowing filtering, concentration and analysis of peptides from a complex mixture. The platform is composed of a microfluidic filtering device and a superhydrophobic surface integrating surface enhanced Raman scattering (SERS) sensors. The microfluidic device was used to filter specific peptides (MW 1553.73 D) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancers, from albumin (66.5 KD), the most represented protein in human plasma. The filtering process consisted of driving the complex mixture through a porous membrane having a cut-off of 12-14 kD by hydrodynamic flow. The filtered samples coming out of the microfluidic device were subsequently deposited on a superhydrophobic surface formed by micro pillars on top of which nanograins were fabricated. The nanograins coupled to a Raman spectroscopy instrument acted as a SERS sensor and allowed analysis of the filtered sample on top of the surface once it evaporated. By using the presented platform, we demonstrate being able to sort small peptides from bigger proteins and to detect them by using a label-free technique at a resolution down to 0.1 ng μL-1. The combination of microfluidics and nanotechnology to develop the presented microfluidic platform may give rise to a new generation of biosensors capable of detecting low concentration samples from complex mixtures without the need for any sample pretreatment or labelling. The developed devices could have future applications in the field of early diagnosis of severe illnesses, e.g. early cancer detection. This journal is",

author = "Gerardo Perozziello and Patrizio Candeloro and Gentile, {Francesco T.} and Annalisa Nicastri and Perri, {Angela Mena} and Coluccio, {Maria Laura} and A. Adamo and Francesca Pardeo and Rossella Catalano and Elvira Parrotta and Espinosa, {Horacio Dante} and Giovanni Cuda and {Di Fabrizio}, {Enzo M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project {"}New Frontiers in Plasmonic Nanosensing{"} (Grant no. 2011-0338) the projects PON01_02834 {"}Prometeo{"}, PONa3_00435 {"}Biomedpark@UMG{"} and FIRB {"}ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet{"} (cod. RBPR05JH2P_010, CUPB41J09000110005) financed from the Ministry of Education and Research, the project for Young researchers financed from the Ministry of Health {"}High Troughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices{"} (CUP J65C13001350001, project no. GR-2010-2311677) and Cancer biomarker detection using micro-structured/superhydrophobic surfaces and advanced spectroscopy techniques (CUP J65C13001370001, project no. GR-2010-2320665) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.",

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doi = "10.1039/c4ra10486b",

language = "English (US)",

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T1 - Microfluidics & nanotechnology: Towards fully integrated analytical devices for the detection of cancer biomarkers

AU - Perozziello, Gerardo

AU - Candeloro, Patrizio

AU - Gentile, Francesco T.

AU - Nicastri, Annalisa

AU - Perri, Angela Mena

AU - Coluccio, Maria Laura

AU - Adamo, A.

AU - Pardeo, Francesca

AU - Catalano, Rossella

AU - Parrotta, Elvira

AU - Espinosa, Horacio Dante

AU - Cuda, Giovanni

AU - Di Fabrizio, Enzo M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338) the projects PON01_02834 "Prometeo", PONa3_00435 "Biomedpark@UMG" and FIRB "ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod. RBPR05JH2P_010, CUPB41J09000110005) financed from the Ministry of Education and Research, the project for Young researchers financed from the Ministry of Health "High Troughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) and Cancer biomarker detection using micro-structured/superhydrophobic surfaces and advanced spectroscopy techniques (CUP J65C13001370001, project no. GR-2010-2320665) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.

PY - 2014

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Microfluidics & nanotechnology: Towards fully integrated analytical devices for the detection of cancer biomarkers

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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