Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

Gerardo Perozziello; Patrizio Candeloro; Antonio De Grazia; Francesco Esposito; Marco Allione; Maria Laura Coluccio; Rossana Tallerico; Immanuel Valpapuram; Luca Tirinato; Gobind Das; Andrea Giugni; Bruno Torre; Pierangelo Veltri; Ulrich Kruhne; Giuseppe Della Valle; Enzo M. Di Fabrizio

doi:10.1364/oe.24.00a180

Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

Gerardo Perozziello, Patrizio Candeloro, Antonio De Grazia, Francesco Esposito, Marco Allione, Maria Laura Coluccio, Rossana Tallerico, Immanuel Valpapuram, Luca Tirinato, Gobind Das, Andrea Giugni, Bruno Torre, Pierangelo Veltri, Ulrich Kruhne, Giuseppe Della Valle, Enzo M. Di Fabrizio

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.

Original language	English (US)
Pages (from-to)	A180
Journal	Optics Express
Volume	24
Issue number	2
DOIs	https://doi.org/10.1364/oe.24.00a180
State	Published - Dec 11 2015

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338), the project for Young researchers financed from the Ministry of Health "High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University "Magna Graecia" of Catanzaro.

Access to Document

10.1364/oe.24.00a180

Cite this

Perozziello, G., Candeloro, P., De Grazia, A., Esposito, F., Allione, M., Coluccio, M. L., Tallerico, R., Valpapuram, I., Tirinato, L., Das, G., Giugni, A., Torre, B., Veltri, P., Kruhne, U., Della Valle, G., & Di Fabrizio, E. M. (2015). Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers. Optics Express, 24(2), A180. https://doi.org/10.1364/oe.24.00a180

@article{0f2926fb8fa14317ad1929dedf6052e3,

title = "Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers",

abstract = "In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). {\textcopyright} 2015 Optical Society of America.",

author = "Gerardo Perozziello and Patrizio Candeloro and {De Grazia}, Antonio and Francesco Esposito and Marco Allione and Coluccio, {Maria Laura} and Rossana Tallerico and Immanuel Valpapuram and Luca Tirinato and Gobind Das and Andrea Giugni and Bruno Torre and Pierangelo Veltri and Ulrich Kruhne and {Della Valle}, Giuseppe and {Di Fabrizio}, {Enzo M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project {"}New Frontiers in Plasmonic Nanosensing{"} (Grant no. 2011-0338), the project for Young researchers financed from the Ministry of Health {"}High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices{"} (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University {"}Magna Graecia{"} of Catanzaro.",

year = "2015",

month = dec,

day = "11",

doi = "10.1364/oe.24.00a180",

language = "English (US)",

volume = "24",

pages = "A180",

journal = "Optics Express",

issn = "1094-4087",

publisher = "Optica Publishing Group",

number = "2",

}

Perozziello, G, Candeloro, P, De Grazia, A, Esposito, F, Allione, M, Coluccio, ML, Tallerico, R, Valpapuram, I, Tirinato, L, Das, G, Giugni, A, Torre, B, Veltri, P, Kruhne, U, Della Valle, G & Di Fabrizio, EM 2015, 'Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers', Optics Express, vol. 24, no. 2, pp. A180. https://doi.org/10.1364/oe.24.00a180

TY - JOUR

T1 - Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

AU - Perozziello, Gerardo

AU - Candeloro, Patrizio

AU - De Grazia, Antonio

AU - Esposito, Francesco

AU - Allione, Marco

AU - Coluccio, Maria Laura

AU - Tallerico, Rossana

AU - Valpapuram, Immanuel

AU - Tirinato, Luca

AU - Das, Gobind

AU - Giugni, Andrea

AU - Torre, Bruno

AU - Veltri, Pierangelo

AU - Kruhne, Ulrich

AU - Della Valle, Giuseppe

AU - Di Fabrizio, Enzo M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338), the project for Young researchers financed from the Ministry of Health "High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University "Magna Graecia" of Catanzaro.

PY - 2015/12/11

Y1 - 2015/12/11

N2 - In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.

AB - In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.

UR - http://hdl.handle.net/10754/621445

UR - https://www.osapublishing.org/abstract.cfm?URI=oe-24-2-A180

UR - http://www.scopus.com/inward/record.url?scp=84962090378&partnerID=8YFLogxK

U2 - 10.1364/oe.24.00a180

DO - 10.1364/oe.24.00a180

M3 - Article

SN - 1094-4087

VL - 24

SP - A180

JO - Optics Express

JF - Optics Express

IS - 2

ER -

Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this