Microbe-induced drought tolerance by ABA-mediated root architecture and epigenetic reprogramming

Khairiah M. Alwutayd; Anamika A. Rawat; Arsheed H. Sheikh; Marilia Almeida-Trapp; Alaguraj Veluchamy; Rewaa Jalal; Michael Karampelias; Katja Froehlich; Waad Alzaed; Naheed Tabassum; Thayssa Rabelo Schley; Anton R. Schäffner; Ihsanullah Daur; Maged M. Saad; Heribert Hirt

doi:10.15252/embr.202256754

Microbe-induced drought tolerance by ABA-mediated root architecture and epigenetic reprogramming

Khairiah M. Alwutayd, Anamika A. Rawat, Arsheed H. Sheikh, Marilia Almeida-Trapp, Alaguraj Veluchamy, Rewaa Jalal, Michael Karampelias, Katja Froehlich, Waad Alzaed, Naheed Tabassum, Thayssa Rabelo Schley, Anton R. Schäffner, Ihsanullah Daur, Maged M. Saad, Heribert Hirt^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The use of beneficial microbes to mitigate drought stress tolerance of plants is of great potential albeit little understood. We show here that a root endophytic desert bacterium, Pseudomonas argentinensis strain SA190, enhances drought stress tolerance in Arabidopsis. Transcriptome and genetic analysis demonstrate that SA190-induced root morphogenesis and gene expression is mediated via the plant abscisic acid (ABA) pathway. Moreover, we demonstrate that SA190 primes the promoters of target genes in an epigenetic ABA-dependent manner. Application of SA190 priming on crops is demonstrated for alfalfa, showing enhanced performance under drought conditions. In summary, a single beneficial root bacterial strain can help plants to resist drought conditions.

Original language	English (US)
Article number	e56754
Journal	EMBO reports
Volume	24
Issue number	8
DOIs	https://doi.org/10.15252/embr.202256754
State	Published - Aug 3 2023

Bibliographical note

Funding Information:
The work was supported by a grant to HH from the King Abdullah University of Science and Technology BAS/1/1062‐01‐01. We thank Pedro Rodriguez for providing and q mutants. We thank KAUST core lab facility for RNA seq and hormone measurement processes. We thank Dr. Sabiha Parveen for helping with data uploading. We would like to thank all members of the Hirt lab for useful discussion. aba2‐1 pylpyr

Publisher Copyright:
© 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.

Keywords

ABA
drought stress
H3K4me3
microbiome
water use efficiency (WUE)

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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10.15252/embr.202256754

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Alwutayd, K. M., Rawat, A. A., Sheikh, A. H., Almeida-Trapp, M., Veluchamy, A., Jalal, R., Karampelias, M., Froehlich, K., Alzaed, W., Tabassum, N., Schley, T. R., Schäffner, A. R., Daur, I., Saad, M. M., & Hirt, H. (2023). Microbe-induced drought tolerance by ABA-mediated root architecture and epigenetic reprogramming. EMBO reports, 24(8), Article e56754. https://doi.org/10.15252/embr.202256754

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abstract = "The use of beneficial microbes to mitigate drought stress tolerance of plants is of great potential albeit little understood. We show here that a root endophytic desert bacterium, Pseudomonas argentinensis strain SA190, enhances drought stress tolerance in Arabidopsis. Transcriptome and genetic analysis demonstrate that SA190-induced root morphogenesis and gene expression is mediated via the plant abscisic acid (ABA) pathway. Moreover, we demonstrate that SA190 primes the promoters of target genes in an epigenetic ABA-dependent manner. Application of SA190 priming on crops is demonstrated for alfalfa, showing enhanced performance under drought conditions. In summary, a single beneficial root bacterial strain can help plants to resist drought conditions.",

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author = "Alwutayd, {Khairiah M.} and Rawat, {Anamika A.} and Sheikh, {Arsheed H.} and Marilia Almeida-Trapp and Alaguraj Veluchamy and Rewaa Jalal and Michael Karampelias and Katja Froehlich and Waad Alzaed and Naheed Tabassum and Schley, {Thayssa Rabelo} and Sch{\"a}ffner, {Anton R.} and Ihsanullah Daur and Saad, {Maged M.} and Heribert Hirt",

note = "Funding Information: The work was supported by a grant to HH from the King Abdullah University of Science and Technology BAS/1/1062‐01‐01. We thank Pedro Rodriguez for providing and q mutants. We thank KAUST core lab facility for RNA seq and hormone measurement processes. We thank Dr. Sabiha Parveen for helping with data uploading. We would like to thank all members of the Hirt lab for useful discussion. aba2‐1 pylpyr Publisher Copyright: {\textcopyright} 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.",

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Alwutayd, KM, Rawat, AA , Sheikh, AH , Almeida-Trapp, M, Veluchamy, A, Jalal, R, Karampelias, M, Froehlich, K, Alzaed, W, Tabassum, N, Schley, TR, Schäffner, AR, Daur, I, Saad, MM & Hirt, H 2023, 'Microbe-induced drought tolerance by ABA-mediated root architecture and epigenetic reprogramming', EMBO reports, vol. 24, no. 8, e56754. https://doi.org/10.15252/embr.202256754

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AU - Alwutayd, Khairiah M.

AU - Rawat, Anamika A.

AU - Sheikh, Arsheed H.

AU - Almeida-Trapp, Marilia

AU - Veluchamy, Alaguraj

AU - Jalal, Rewaa

AU - Karampelias, Michael

AU - Froehlich, Katja

AU - Alzaed, Waad

AU - Tabassum, Naheed

AU - Schley, Thayssa Rabelo

AU - Schäffner, Anton R.

AU - Daur, Ihsanullah

AU - Saad, Maged M.

AU - Hirt, Heribert

N1 - Funding Information: The work was supported by a grant to HH from the King Abdullah University of Science and Technology BAS/1/1062‐01‐01. We thank Pedro Rodriguez for providing and q mutants. We thank KAUST core lab facility for RNA seq and hormone measurement processes. We thank Dr. Sabiha Parveen for helping with data uploading. We would like to thank all members of the Hirt lab for useful discussion. aba2‐1 pylpyr Publisher Copyright: © 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.

PY - 2023/8/3

Y1 - 2023/8/3

N2 - The use of beneficial microbes to mitigate drought stress tolerance of plants is of great potential albeit little understood. We show here that a root endophytic desert bacterium, Pseudomonas argentinensis strain SA190, enhances drought stress tolerance in Arabidopsis. Transcriptome and genetic analysis demonstrate that SA190-induced root morphogenesis and gene expression is mediated via the plant abscisic acid (ABA) pathway. Moreover, we demonstrate that SA190 primes the promoters of target genes in an epigenetic ABA-dependent manner. Application of SA190 priming on crops is demonstrated for alfalfa, showing enhanced performance under drought conditions. In summary, a single beneficial root bacterial strain can help plants to resist drought conditions.

AB - The use of beneficial microbes to mitigate drought stress tolerance of plants is of great potential albeit little understood. We show here that a root endophytic desert bacterium, Pseudomonas argentinensis strain SA190, enhances drought stress tolerance in Arabidopsis. Transcriptome and genetic analysis demonstrate that SA190-induced root morphogenesis and gene expression is mediated via the plant abscisic acid (ABA) pathway. Moreover, we demonstrate that SA190 primes the promoters of target genes in an epigenetic ABA-dependent manner. Application of SA190 priming on crops is demonstrated for alfalfa, showing enhanced performance under drought conditions. In summary, a single beneficial root bacterial strain can help plants to resist drought conditions.

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KW - drought stress

KW - H3K4me3

KW - microbiome

KW - water use efficiency (WUE)

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SN - 1469-221X

VL - 24

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ER -

Microbe-induced drought tolerance by ABA-mediated root architecture and epigenetic reprogramming

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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