Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula

Gareth A Pearson; Asuncion Lago-Leston; Fernando Cánovas; Cymon J Cox; Frederic Verret; Sebastian Lasternas; Carlos M. Duarte; Susana Agusti; Ester A Serrão

doi:10.1038/ismej.2015.40

Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula

Gareth A Pearson, Asuncion Lago-Leston, Fernando Cánovas, Cymon J Cox, Frederic Verret, Sebastian Lasternas, Carlos M. Duarte, Susana Agusti, Ester A Serrão

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Abstract

Functional genomics of diatom-dominated communities fromthe Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the Bellingshausen Sea/Wilkins Ice Shelf yielded more than 500K pyrosequencing reads that were combined to produce a global metatranscriptome assembly. Multi-gene phylogenies recovered three distinct communities, and diatom-assigned contigs further indicated little read-sharing between communities, validating an assembly-based annotation and analysis approach. Although functional analysis recovered a core of abundant shared annotations that were expressed across the three diatom communities, over 40% of annotations (but accounting for

Original language	English (US)
Pages (from-to)	2275-2289
Number of pages	15
Journal	The ISME Journal
Volume	9
Issue number	10
DOIs	https://doi.org/10.1038/ismej.2015.40
State	Published - Apr 14 2015

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abstract = "Functional genomics of diatom-dominated communities fromthe Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the Bellingshausen Sea/Wilkins Ice Shelf yielded more than 500K pyrosequencing reads that were combined to produce a global metatranscriptome assembly. Multi-gene phylogenies recovered three distinct communities, and diatom-assigned contigs further indicated little read-sharing between communities, validating an assembly-based annotation and analysis approach. Although functional analysis recovered a core of abundant shared annotations that were expressed across the three diatom communities, over 40% of annotations (but accounting for",

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AU - Pearson, Gareth A

AU - Lago-Leston, Asuncion

AU - Cánovas, Fernando

AU - Cox, Cymon J

AU - Verret, Frederic

AU - Lasternas, Sebastian

AU - Duarte, Carlos M.

AU - Agusti, Susana

AU - Serrão, Ester A

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PY - 2015/4/14

Y1 - 2015/4/14

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AB - Functional genomics of diatom-dominated communities fromthe Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the Bellingshausen Sea/Wilkins Ice Shelf yielded more than 500K pyrosequencing reads that were combined to produce a global metatranscriptome assembly. Multi-gene phylogenies recovered three distinct communities, and diatom-assigned contigs further indicated little read-sharing between communities, validating an assembly-based annotation and analysis approach. Although functional analysis recovered a core of abundant shared annotations that were expressed across the three diatom communities, over 40% of annotations (but accounting for

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UR - http://www.nature.com/articles/ismej201540

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JF - The ISME Journal

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Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula

Abstract

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