Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula

Gareth A Pearson, Asuncion Lago-Leston, Fernando Cánovas, Cymon J Cox, Frederic Verret, Sebastian Lasternas, Carlos M. Duarte, Susana Agusti, Ester A Serrão

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33 Scopus citations


Functional genomics of diatom-dominated communities fromthe Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the Bellingshausen Sea/Wilkins Ice Shelf yielded more than 500K pyrosequencing reads that were combined to produce a global metatranscriptome assembly. Multi-gene phylogenies recovered three distinct communities, and diatom-assigned contigs further indicated little read-sharing between communities, validating an assembly-based annotation and analysis approach. Although functional analysis recovered a core of abundant shared annotations that were expressed across the three diatom communities, over 40% of annotations (but accounting for
Original languageEnglish (US)
Pages (from-to)2275-2289
Number of pages15
JournalThe ISME Journal
Issue number10
StatePublished - Apr 14 2015

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KAUST Repository Item: Exported on 2020-10-01


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