Metagenomics-based evaluation of groundwater microbial profiles in response to treated wastewater discharge

Noor A. Zaouri, Muhammad Raihan Jumat, Tariq Cheema, Pei-Ying Hong

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


This study aims to demonstrate the use of metagenomics to assess groundwater quality. Metagenomics revealed a lower alpha diversity for both bacteria and virus in wastewater-exposed groundwater compared to the upstream controls. An increase in the relative abundance of Planctomycetes and Picornaviridae was also observed in wastewater-exposed groundwater. However, comparison of antibiotic resistome cannot clearly differentiate wastewater-exposed groundwater from control. Findings suggest that metagenomics can detect selected microbial signatures indicative of treated wastewater discharge.
Original languageEnglish (US)
Pages (from-to)108835
JournalEnvironmental Research
StatePublished - Oct 18 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): BAS/1/1033-01-01
Acknowledgements: The authors would like to thank Dr Moustapha Harb and Dhafer Alsaleh for assistance during the early sampling years. The authors would also like to thank Professor Tom Missimer and Mr. Mohammed AlRayaan for providing background information related to the hydrogeological characteristics of Wadi Fatimah. This study is funded by KAUST Baseline Grant BAS/1/1033-01-01 awarded to P.-Y. Hong.


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