Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses.

Bahget Talat Hamooh, Farooq Abdul Sattar, Gordon Wellman, Magdi Ali Ahmed Mousa

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22 Scopus citations


Globally, many crop production areas are threatened by drought and salinity. Potato ($\textit{Solanum tuberosum}$ L.) is susceptible to these challenging environmental conditions. In this study, an in vitro approach was employed to compare the tolerance of potato cultivars 'BARI-401' (red skin) and 'Spunta' (yellow skin). To simulate ionic and osmotic stress, MS media was supplemented with lithium chloride (LiCl 20 mM) and mannitol (150 mM). GC-MS and spectrophotometry techniques were used to determine metabolite accumulation. Other biochemical properties, such as total phenols concentration (TPC), total flavonoids concentration (TFC), antioxidant capacity (DPPH free radical scavenging capacity), polyphenol oxidase (PPO), and peroxidase (POD) activities, were also measured. The two cultivars respond differently to ionic and osmotic stress treatments, with Spunta accumulating more defensive metabolites in response, indicating a higher level of tolerance. While further investigation of the physiological and biochemical responses of these varieties to drought and salinity is required, the approach taken in this paper provides useful information prior to open field evaluation.
Original languageEnglish (US)
Pages (from-to)98
JournalPlants (Basel, Switzerland)
Issue number1
StatePublished - Jan 9 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-01-12
Acknowledgements: The authors are grateful to the Deanship of Graduate Studies (DGS), king Abdulaziz University, for endowment of the Ph.D. scholarship under student ID. No 1801819. Moreover, the authors acknowledge the moral and technical support from the Department of Arid Land Agriculture.


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