Metabolite and matrix interference in phenytoin immunoassays

Petrie M. Rainey; Katherine E. Rogers; William L. Roberts

doi:10.1093/clinchem/42.10.1645

Metabolite and matrix interference in phenytoin immunoassays

Petrie M. Rainey, Katherine E. Rogers, William L. Roberts

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The major phenytoin metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin glucuronide (HPPG), was primarily responsible for the positive bias noted when uremic specimens were assayed with the Abbott TDx® Free Phenytoin fluorescence polarization immunoassay. The amount of bias depended on both HPPG and phenytoin concentration, increasing with increases in either concentration. The new Abbott TDx II assays for phenytoin and free phenytoin exhibited no significant cross-reactivity with HPPG and no bias in clinical specimens from uremic patients. Both assays correlated well with Emit-based assays (r >0.98), had CVs of

Original language	English (US)
Pages (from-to)	1645-1653
Number of pages	9
Journal	Clinical Chemistry
Volume	42
Issue number	10
DOIs	https://doi.org/10.1093/clinchem/42.10.1645
State	Published - Jan 1 1996
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-20

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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10.1093/clinchem/42.10.1645

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abstract = "The major phenytoin metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin glucuronide (HPPG), was primarily responsible for the positive bias noted when uremic specimens were assayed with the Abbott TDx{\textregistered} Free Phenytoin fluorescence polarization immunoassay. The amount of bias depended on both HPPG and phenytoin concentration, increasing with increases in either concentration. The new Abbott TDx II assays for phenytoin and free phenytoin exhibited no significant cross-reactivity with HPPG and no bias in clinical specimens from uremic patients. Both assays correlated well with Emit-based assays (r >0.98), had CVs of",

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AU - Rainey, Petrie M.

AU - Rogers, Katherine E.

AU - Roberts, William L.

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N2 - The major phenytoin metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin glucuronide (HPPG), was primarily responsible for the positive bias noted when uremic specimens were assayed with the Abbott TDx® Free Phenytoin fluorescence polarization immunoassay. The amount of bias depended on both HPPG and phenytoin concentration, increasing with increases in either concentration. The new Abbott TDx II assays for phenytoin and free phenytoin exhibited no significant cross-reactivity with HPPG and no bias in clinical specimens from uremic patients. Both assays correlated well with Emit-based assays (r >0.98), had CVs of

AB - The major phenytoin metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin glucuronide (HPPG), was primarily responsible for the positive bias noted when uremic specimens were assayed with the Abbott TDx® Free Phenytoin fluorescence polarization immunoassay. The amount of bias depended on both HPPG and phenytoin concentration, increasing with increases in either concentration. The new Abbott TDx II assays for phenytoin and free phenytoin exhibited no significant cross-reactivity with HPPG and no bias in clinical specimens from uremic patients. Both assays correlated well with Emit-based assays (r >0.98), had CVs of

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Metabolite and matrix interference in phenytoin immunoassays

Abstract

Bibliographical note

ASJC Scopus subject areas

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