Abstract
BACKGROUND: Measurement of serum thyroglobulin (Tg) is used to monitor patients after treatment for differentiated thyroid carcinoma (TC). Difficulty in using Tg as a biomarker of the recurrence of TC in many patients stems from the presence of endogenous anti-Tg autoantibodies (Tg-AAbs), which can interfere with immunoassays (IAs) and cause false-negative results. METHODS: We enriched Tg from serum samples using rabbit polyclonal anti-Tg antiserum and protein precipitation. Unrelated proteins were partially depleted in the process. Enriched proteins were then denatured, reduced, and digested with trypsin after the addition of a winged internal standard peptide. A Tg-specific tryptic peptide was purified by immunoaffinity extraction and analyzed by 2-dimensional LC-MS/MS. Instrument cycle time was 6.5 min per sample. RESULTS: The lower limit of quantification was 0.5 ng/mL (0.76 fmol/mL dimer). Total imprecision of triplicate measurements in serum samples over 5 days was
Original language | English (US) |
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Pages (from-to) | 982-990 |
Number of pages | 9 |
Journal | Clinical Chemistry |
Volume | 59 |
Issue number | 6 |
DOIs | |
State | Published - Jun 1 2013 |
Bibliographical note
Generated from Scopus record by KAUST IRTS on 2023-09-20ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical