TY - JOUR
T1 - Mannosylated liposomes for targeted vaccines delivery.
AU - Vyas, Suresh Prasad
AU - Goyal, Amit K.
AU - Khatri, Kapil
N1 - Generated from Scopus record by KAUST IRTS on 2023-10-12
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Mannosylated liposomes appear to be a promising and potential carrier system for delivery of proteins, peptides, or nucleic acids. The present chapter describes novel mannosylated liposomes, which increase the intracellular targeting of immunogen to dendritic cells and macrophages possessing the specific receptors. The liposomes used in the present investigation were prepared by hand-shaken method and characterized for size, shape, surface charge, encapsulation efficiency, ligand binding, and specificity and uptake studies. The immune-stimulating activity of the liposomes was studied by measuring antigen-specific antibody titer following subcutaneous administration of different liposomal formulations in BALB/c mice. It was found that O-palmitoyl mannan (OPM)-coated liposomes showed better uptake efficiency. In vivo studies revealed that the OPM-coated liposomes exhibited significant higher serum antibody response and stronger TH1/TH2-based cellular responses. In conclusion, novel vesicular constructs are useful nanosized carriers having superior surface characteristics--for active interaction with the antigen-presenting cells and subsequent processing and presentation of antigen.
AB - Mannosylated liposomes appear to be a promising and potential carrier system for delivery of proteins, peptides, or nucleic acids. The present chapter describes novel mannosylated liposomes, which increase the intracellular targeting of immunogen to dendritic cells and macrophages possessing the specific receptors. The liposomes used in the present investigation were prepared by hand-shaken method and characterized for size, shape, surface charge, encapsulation efficiency, ligand binding, and specificity and uptake studies. The immune-stimulating activity of the liposomes was studied by measuring antigen-specific antibody titer following subcutaneous administration of different liposomal formulations in BALB/c mice. It was found that O-palmitoyl mannan (OPM)-coated liposomes showed better uptake efficiency. In vivo studies revealed that the OPM-coated liposomes exhibited significant higher serum antibody response and stronger TH1/TH2-based cellular responses. In conclusion, novel vesicular constructs are useful nanosized carriers having superior surface characteristics--for active interaction with the antigen-presenting cells and subsequent processing and presentation of antigen.
UR - http://link.springer.com/10.1007/978-1-60327-360-2_12
UR - http://www.scopus.com/inward/record.url?scp=77950467090&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-360-2_12
DO - 10.1007/978-1-60327-360-2_12
M3 - Article
SN - 1940-6029
VL - 605
SP - 177
EP - 188
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -
