Low concentration protein detection using novel SERS devices

Gobind Das, Francesco Gentile, Maria Laura Coluccio, G. Cojoc, Federico Mecarini, Francesco De Angelis, Patrizio Candeloro, Carlo Liberale, Enzo Di Fabrizio*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations


We report, herein, novel processes of nanofabrication techniques for few molecules detection by generating surface plasmons, thus giving a giant electric field in a controllable and reproducible manner. Surface enhanced Raman scattering (SERS) measurements are performed for different proteins, namely, lysozyme, ribonuclease-B, bovin serum albumin, ferritin, and myoglobin in the temperature range between -65°C and 90°C, using Device1, which is fabricated by means of e-beam and electro-plating technique. The calculation shows the possible detection of myoglobin concentration down to attomole. The in-depth analysis even for small conformational changes is performed using 2D Raman correlation analysis and difference Raman analysis in order to gain straightforward understanding of proteins undergoing thermodynamical perturbation. Device2 is fabricated by e-beam and metal electroless techniques to investigate the Rhodamine 6G (R6G) of different concentrations. Modifying this device by using bimetal (Ag and Au) electroless deposition permits us the further enhancement in Raman signal and better durability.

Original languageEnglish (US)
Title of host publicationOptical Fluorescence Microscopy
Subtitle of host publicationFrom the Spectral to the Nano Dimension
PublisherSpringer-Verlag Berlin Heidelberg
Number of pages20
ISBN (Electronic)9783642151750
ISBN (Print)9783642151743
StatePublished - Jan 1 2011

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Physics and Astronomy(all)


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