Low concentration protein detection using novel SERS devices

Gobind Das; Francesco Gentile; Maria Laura Coluccio; G. Cojoc; Federico Mecarini; Francesco De Angelis; Patrizio Candeloro; Carlo Liberale; Enzo Di Fabrizio

doi:10.1007/978-3-642-15175-0_12

Low concentration protein detection using novel SERS devices

Gobind Das, Francesco Gentile, Maria Laura Coluccio, G. Cojoc, Federico Mecarini, Francesco De Angelis, Patrizio Candeloro, Carlo Liberale, Enzo Di Fabrizio^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

We report, herein, novel processes of nanofabrication techniques for few molecules detection by generating surface plasmons, thus giving a giant electric field in a controllable and reproducible manner. Surface enhanced Raman scattering (SERS) measurements are performed for different proteins, namely, lysozyme, ribonuclease-B, bovin serum albumin, ferritin, and myoglobin in the temperature range between -65°C and 90°C, using Device1, which is fabricated by means of e-beam and electro-plating technique. The calculation shows the possible detection of myoglobin concentration down to attomole. The in-depth analysis even for small conformational changes is performed using 2D Raman correlation analysis and difference Raman analysis in order to gain straightforward understanding of proteins undergoing thermodynamical perturbation. Device2 is fabricated by e-beam and metal electroless techniques to investigate the Rhodamine 6G (R6G) of different concentrations. Modifying this device by using bimetal (Ag and Au) electroless deposition permits us the further enhancement in Raman signal and better durability.

Original language	English (US)
Title of host publication	Optical Fluorescence Microscopy
Subtitle of host publication	From the Spectral to the Nano Dimension
Publisher	Springer-Verlag Berlin Heidelberg
Pages	191-210
Number of pages	20
Volume	9783642151750
ISBN (Electronic)	9783642151750
ISBN (Print)	9783642151743
DOIs	https://doi.org/10.1007/978-3-642-15175-0_12
State	Published - 2011
Externally published	Yes

Bibliographical note

Publisher Copyright:
© Springer-Verlag Berlin Heidelberg 2011. All rights are reserved.

ASJC Scopus subject areas

General Agricultural and Biological Sciences
General Biochemistry, Genetics and Molecular Biology
General Medicine
General Physics and Astronomy

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10.1007/978-3-642-15175-0_12

Cite this

Das, G., Gentile, F., Coluccio, M. L., Cojoc, G., Mecarini, F., De Angelis, F., Candeloro, P., Liberale, C., & Di Fabrizio, E. (2011). Low concentration protein detection using novel SERS devices. In Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension (Vol. 9783642151750, pp. 191-210). Springer-Verlag Berlin Heidelberg. https://doi.org/10.1007/978-3-642-15175-0_12

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abstract = "We report, herein, novel processes of nanofabrication techniques for few molecules detection by generating surface plasmons, thus giving a giant electric field in a controllable and reproducible manner. Surface enhanced Raman scattering (SERS) measurements are performed for different proteins, namely, lysozyme, ribonuclease-B, bovin serum albumin, ferritin, and myoglobin in the temperature range between -65°C and 90°C, using Device1, which is fabricated by means of e-beam and electro-plating technique. The calculation shows the possible detection of myoglobin concentration down to attomole. The in-depth analysis even for small conformational changes is performed using 2D Raman correlation analysis and difference Raman analysis in order to gain straightforward understanding of proteins undergoing thermodynamical perturbation. Device2 is fabricated by e-beam and metal electroless techniques to investigate the Rhodamine 6G (R6G) of different concentrations. Modifying this device by using bimetal (Ag and Au) electroless deposition permits us the further enhancement in Raman signal and better durability.",

author = "Gobind Das and Francesco Gentile and Coluccio, {Maria Laura} and G. Cojoc and Federico Mecarini and {De Angelis}, Francesco and Patrizio Candeloro and Carlo Liberale and {Di Fabrizio}, Enzo",

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doi = "10.1007/978-3-642-15175-0_12",

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Das, G, Gentile, F, Coluccio, ML, Cojoc, G, Mecarini, F, De Angelis, F, Candeloro, P, Liberale, C & Di Fabrizio, E 2011, Low concentration protein detection using novel SERS devices. in Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension. vol. 9783642151750, Springer-Verlag Berlin Heidelberg, pp. 191-210. https://doi.org/10.1007/978-3-642-15175-0_12

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T1 - Low concentration protein detection using novel SERS devices

AU - Das, Gobind

AU - Gentile, Francesco

AU - Coluccio, Maria Laura

AU - Cojoc, G.

AU - Mecarini, Federico

AU - De Angelis, Francesco

AU - Candeloro, Patrizio

AU - Liberale, Carlo

AU - Di Fabrizio, Enzo

PY - 2011

Y1 - 2011

N2 - We report, herein, novel processes of nanofabrication techniques for few molecules detection by generating surface plasmons, thus giving a giant electric field in a controllable and reproducible manner. Surface enhanced Raman scattering (SERS) measurements are performed for different proteins, namely, lysozyme, ribonuclease-B, bovin serum albumin, ferritin, and myoglobin in the temperature range between -65°C and 90°C, using Device1, which is fabricated by means of e-beam and electro-plating technique. The calculation shows the possible detection of myoglobin concentration down to attomole. The in-depth analysis even for small conformational changes is performed using 2D Raman correlation analysis and difference Raman analysis in order to gain straightforward understanding of proteins undergoing thermodynamical perturbation. Device2 is fabricated by e-beam and metal electroless techniques to investigate the Rhodamine 6G (R6G) of different concentrations. Modifying this device by using bimetal (Ag and Au) electroless deposition permits us the further enhancement in Raman signal and better durability.

AB - We report, herein, novel processes of nanofabrication techniques for few molecules detection by generating surface plasmons, thus giving a giant electric field in a controllable and reproducible manner. Surface enhanced Raman scattering (SERS) measurements are performed for different proteins, namely, lysozyme, ribonuclease-B, bovin serum albumin, ferritin, and myoglobin in the temperature range between -65°C and 90°C, using Device1, which is fabricated by means of e-beam and electro-plating technique. The calculation shows the possible detection of myoglobin concentration down to attomole. The in-depth analysis even for small conformational changes is performed using 2D Raman correlation analysis and difference Raman analysis in order to gain straightforward understanding of proteins undergoing thermodynamical perturbation. Device2 is fabricated by e-beam and metal electroless techniques to investigate the Rhodamine 6G (R6G) of different concentrations. Modifying this device by using bimetal (Ag and Au) electroless deposition permits us the further enhancement in Raman signal and better durability.

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PB - Springer-Verlag Berlin Heidelberg

ER -

Low concentration protein detection using novel SERS devices

Abstract

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