Low activity of lytic pelagiphages in coastal marine waters

Laura Alonso-Sáez; Xosé Anxelu G. Morán; Martha Rj Clokie

doi:10.1038/s41396-018-0185-y

Low activity of lytic pelagiphages in coastal marine waters

Laura Alonso-Sáez^*, Xosé Anxelu G. Morán, Martha Rj Clokie

^*Corresponding author for this work

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Phages infect marine bacteria impacting their dynamics, diversity and physiology, but little is known about specific phage-host interactions in situ. We analyzed the joint dynamics in the abundance of phage-related transcripts, as an indicator of viral lytic activity, and their potential hosts using a metatranscriptomic dataset obtained over 2 years in coastal temperate waters of the NE Atlantic. Substantial temporal variability was identified in the expression levels of different phages, likely in response to host availability. Indeed, a significant positive relationship between the abundance of transcripts from some of the most abundant phage types (infecting SAR11, SAR116 and cyanobacteria) and their putative hosts was found. Yet, the ratio of increase in phage transcripts per host cell was significantly lower for pelagiphages than for the HMO-2011 phage, which infects SAR116. Despite the high abundance of pelagiphages in the ocean, they may be less active than other phage types in coastal waters.

Original language	English (US)
Pages (from-to)	2100-2102
Number of pages	3
Journal	ISME Journal
Volume	12
Issue number	8
DOIs	https://doi.org/10.1038/s41396-018-0185-y
State	Published - Aug 1 2018

Bibliographical note

Funding Information:
Acknowledgements We are grateful to the captain and crew of RV ‘José de Rioja’ and to the colleagues from IEO Xixón for their help with RNA samples collection, especially to N. Arandia-Gorostidi and T. Huete-Stauffer. Bioinformatic analyses have been supported by CESGA (Centro de Supercomputación de Galicia) and ALICE HPC (University of Leicester). This work was funded by the Marie Curie European Reintegration Grant FUNDIVERSITY’ (FP7, Grant Agreement 268331) the British Ecological Society and the time-series program RADIALES from the Spanish Institute of Oceanography (IEO). LA-S was supported by the research contracts ‘Juan de la Cierva’ and ‘Ramon y Cajal’ from the Spanish Ministry of Economy and Competitiveness.

Publisher Copyright:
© 2018 International Society for Microbial Ecology.

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41396-018-0185-y

Cite this

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title = "Low activity of lytic pelagiphages in coastal marine waters",

abstract = "Phages infect marine bacteria impacting their dynamics, diversity and physiology, but little is known about specific phage-host interactions in situ. We analyzed the joint dynamics in the abundance of phage-related transcripts, as an indicator of viral lytic activity, and their potential hosts using a metatranscriptomic dataset obtained over 2 years in coastal temperate waters of the NE Atlantic. Substantial temporal variability was identified in the expression levels of different phages, likely in response to host availability. Indeed, a significant positive relationship between the abundance of transcripts from some of the most abundant phage types (infecting SAR11, SAR116 and cyanobacteria) and their putative hosts was found. Yet, the ratio of increase in phage transcripts per host cell was significantly lower for pelagiphages than for the HMO-2011 phage, which infects SAR116. Despite the high abundance of pelagiphages in the ocean, they may be less active than other phage types in coastal waters.",

author = "Laura Alonso-S{\'a}ez and Mor{\'a}n, {Xos{\'e} Anxelu G.} and Clokie, {Martha Rj}",

note = "Funding Information: Acknowledgements We are grateful to the captain and crew of RV {\textquoteleft}Jos{\'e} de Rioja{\textquoteright} and to the colleagues from IEO Xix{\'o}n for their help with RNA samples collection, especially to N. Arandia-Gorostidi and T. Huete-Stauffer. Bioinformatic analyses have been supported by CESGA (Centro de Supercomputaci{\'o}n de Galicia) and ALICE HPC (University of Leicester). This work was funded by the Marie Curie European Reintegration Grant FUNDIVERSITY{\textquoteright} (FP7, Grant Agreement 268331) the British Ecological Society and the time-series program RADIALES from the Spanish Institute of Oceanography (IEO). LA-S was supported by the research contracts {\textquoteleft}Juan de la Cierva{\textquoteright} and {\textquoteleft}Ramon y Cajal{\textquoteright} from the Spanish Ministry of Economy and Competitiveness. Publisher Copyright: {\textcopyright} 2018 International Society for Microbial Ecology.",

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N2 - Phages infect marine bacteria impacting their dynamics, diversity and physiology, but little is known about specific phage-host interactions in situ. We analyzed the joint dynamics in the abundance of phage-related transcripts, as an indicator of viral lytic activity, and their potential hosts using a metatranscriptomic dataset obtained over 2 years in coastal temperate waters of the NE Atlantic. Substantial temporal variability was identified in the expression levels of different phages, likely in response to host availability. Indeed, a significant positive relationship between the abundance of transcripts from some of the most abundant phage types (infecting SAR11, SAR116 and cyanobacteria) and their putative hosts was found. Yet, the ratio of increase in phage transcripts per host cell was significantly lower for pelagiphages than for the HMO-2011 phage, which infects SAR116. Despite the high abundance of pelagiphages in the ocean, they may be less active than other phage types in coastal waters.

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Low activity of lytic pelagiphages in coastal marine waters

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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