Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals

Mark M. Kushnir, Takara Blamires, Alan L. Rockwood, William L. Roberts, Bingfang Yue, Evrim Erdogan, Ashley M. Bunker, A. Wayne Meikle

Research output: Contribution to journalArticlepeer-review

235 Scopus citations

Abstract

BACKGROUND: Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedione, dehydroepiandrosterone (DHEA), and testosterone in these patients. METHODS: We spiked 200 μL of serum or plasma with isotope-labeled internal standards and performed extraction with methyl t-butyl ether. We then derivatized the extracts with hydroxylamine and analyzed them by LC-MS/MS using a 2-dimensional chromatographic separation with a 3.5-min analysis time. RESULTS: Total imprecision for each analyte was
Original languageEnglish (US)
Pages (from-to)1138-1147
Number of pages10
JournalClinical Chemistry
Volume56
Issue number7
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-20

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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