Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals

Mark M. Kushnir; Takara Blamires; Alan L. Rockwood; William L. Roberts; Bingfang Yue; Evrim Erdogan; Ashley M. Bunker; A. Wayne Meikle

doi:10.1373/clinchem.2010.143222

Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals

Mark M. Kushnir, Takara Blamires, Alan L. Rockwood, William L. Roberts, Bingfang Yue, Evrim Erdogan, Ashley M. Bunker, A. Wayne Meikle

Research output: Contribution to journal › Article › peer-review

240 Scopus citations

Abstract

BACKGROUND: Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedione, dehydroepiandrosterone (DHEA), and testosterone in these patients. METHODS: We spiked 200 μL of serum or plasma with isotope-labeled internal standards and performed extraction with methyl t-butyl ether. We then derivatized the extracts with hydroxylamine and analyzed them by LC-MS/MS using a 2-dimensional chromatographic separation with a 3.5-min analysis time. RESULTS: Total imprecision for each analyte was

Original language	English (US)
Pages (from-to)	1138-1147
Number of pages	10
Journal	Clinical Chemistry
Volume	56
Issue number	7
DOIs	https://doi.org/10.1373/clinchem.2010.143222
State	Published - Jul 1 2010
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-20

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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10.1373/clinchem.2010.143222

Cite this

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title = "Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals",

abstract = "BACKGROUND: Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedione, dehydroepiandrosterone (DHEA), and testosterone in these patients. METHODS: We spiked 200 μL of serum or plasma with isotope-labeled internal standards and performed extraction with methyl t-butyl ether. We then derivatized the extracts with hydroxylamine and analyzed them by LC-MS/MS using a 2-dimensional chromatographic separation with a 3.5-min analysis time. RESULTS: Total imprecision for each analyte was",

author = "Kushnir, {Mark M.} and Takara Blamires and Rockwood, {Alan L.} and Roberts, {William L.} and Bingfang Yue and Evrim Erdogan and Bunker, {Ashley M.} and Meikle, {A. Wayne}",

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year = "2010",

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doi = "10.1373/clinchem.2010.143222",

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T1 - Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals

AU - Kushnir, Mark M.

AU - Blamires, Takara

AU - Rockwood, Alan L.

AU - Roberts, William L.

AU - Yue, Bingfang

AU - Erdogan, Evrim

AU - Bunker, Ashley M.

AU - Meikle, A. Wayne

N1 - Generated from Scopus record by KAUST IRTS on 2023-09-20

PY - 2010/7/1

Y1 - 2010/7/1

N2 - BACKGROUND: Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedione, dehydroepiandrosterone (DHEA), and testosterone in these patients. METHODS: We spiked 200 μL of serum or plasma with isotope-labeled internal standards and performed extraction with methyl t-butyl ether. We then derivatized the extracts with hydroxylamine and analyzed them by LC-MS/MS using a 2-dimensional chromatographic separation with a 3.5-min analysis time. RESULTS: Total imprecision for each analyte was

AB - BACKGROUND: Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedione, dehydroepiandrosterone (DHEA), and testosterone in these patients. METHODS: We spiked 200 μL of serum or plasma with isotope-labeled internal standards and performed extraction with methyl t-butyl ether. We then derivatized the extracts with hydroxylamine and analyzed them by LC-MS/MS using a 2-dimensional chromatographic separation with a 3.5-min analysis time. RESULTS: Total imprecision for each analyte was

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U2 - 10.1373/clinchem.2010.143222

DO - 10.1373/clinchem.2010.143222

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JO - Clinical Chemistry

JF - Clinical Chemistry

IS - 7

ER -

Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals

Abstract

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ASJC Scopus subject areas

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