Liposomes as adjuvant for combination vaccines

Neeraj Mishra, Prem N. Gupta, Sunil Mahor, Kapil Khatri, Amit K. Goyal, Suresh P. Vyas

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


In the present study tetanus toxoid (TT) loaded liposomes and diphtheria toxoid (DT) loaded liposomes were prepared by reverse phase evaporation method and after combining these two vaccines the potential advantages were investigated. Prepared systems were characterized for the size, shape and entrapment efficiency. SDS-PAGE analysis of TT and DT was also performed. The selected liposomal formulations were administered subcutaneously to Balb/c mice and their immune responses were determined using ELISA after 15, 30, 45 days. After boosting the maximum immune response was observed after 45 days and was found to be 0.831 and 0.749 for TT loaded liposome and DT loaded liposomes respectively. When the mice were immunized subcutaneously with the physical mixture of TT loaded liposomes and DT loaded liposomes the immune response for the combination vaccine was found to be 1.44 and 0.741 for the TT and DT respectively. The result showed that the immune response of TT increased when it was combined with DT in liposomes. This confirms adjuvantcity of DT vis-a-vis immunogenicity. Thus, carrier mediated cocktail vaccination holds promise for clinical applications.
Original languageEnglish (US)
Pages (from-to)237-241
Number of pages5
JournalIndian Journal of Experimental Biology
Issue number3
StatePublished - Mar 1 2007
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-10-12

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biotechnology


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