Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites

Josef Tichý; Zdenko Gardian; David Bína; Peter Koník; Radek V. Litvín; Miroslava Herbstová; Arnab Pain; František Vácha

doi:10.1016/j.bbabio.2013.02.002

Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites

Josef Tichý, Zdenko Gardian, David Bína, Peter Koník, Radek V. Litvín, Miroslava Herbstová, Arnab Pain, František Vácha

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32 Scopus citations

Abstract

The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18-19 kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle analysis and circular dichroism spectroscopy indicated similarity of this structure to the recently characterized XLH antenna of xanthophytes. In light of these data we denote this antenna complex CLH, for "Chromera Light Harvesting" complex. The other system was identified as the photosystem I with bound Light Harvesting Complexes (PSI-LHCr) related to the red algae LHCI antennae. The result of this study is the finding that C. velia, when grown in natural light conditions, possesses light harvesting antennae typically found in two different, evolutionary distant, groups of photosynthetic organisms. © 2013 Elsevier B.V. All rights reserved.

Original language	English (US)
Pages (from-to)	723-729
Number of pages	7
Journal	Biochimica et Biophysica Acta (BBA) - Bioenergetics
Volume	1827
Issue number	6
DOIs	https://doi.org/10.1016/j.bbabio.2013.02.002
State	Published - Jun 2013

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): IC/2010/09
Acknowledgements: This research was supported by the GAAV 32-IAA601410907 and GAJU 134/2010/P with institutional support RVO:60077344. Research on C. velia genomics is supported by an Award IC/2010/09 made by the King Abdullah University of Science and Technology (KAUST) to A. Pain. The authors are greatly indebted to the team of J. Lukes, R. Sobotka and P. Keeling, for giving access to their data concerning the split of the photosystem I reaction center protein prior to publication. Thanks are extended to I. Hunalova and F. Matousek for technical assistance.

ASJC Scopus subject areas

Biochemistry
Cell Biology
Biophysics

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10.1016/j.bbabio.2013.02.002

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title = "Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites",

abstract = "The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18-19 kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle analysis and circular dichroism spectroscopy indicated similarity of this structure to the recently characterized XLH antenna of xanthophytes. In light of these data we denote this antenna complex CLH, for {"}Chromera Light Harvesting{"} complex. The other system was identified as the photosystem I with bound Light Harvesting Complexes (PSI-LHCr) related to the red algae LHCI antennae. The result of this study is the finding that C. velia, when grown in natural light conditions, possesses light harvesting antennae typically found in two different, evolutionary distant, groups of photosynthetic organisms. {\textcopyright} 2013 Elsevier B.V. All rights reserved.",

author = "Josef Tich{\'y} and Zdenko Gardian and David B{\'i}na and Peter Kon{\'i}k and Litv{\'i}n, {Radek V.} and Miroslava Herbstov{\'a} and Arnab Pain and Franti{\v s}ek V{\'a}cha",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): IC/2010/09 Acknowledgements: This research was supported by the GAAV 32-IAA601410907 and GAJU 134/2010/P with institutional support RVO:60077344. Research on C. velia genomics is supported by an Award IC/2010/09 made by the King Abdullah University of Science and Technology (KAUST) to A. Pain. The authors are greatly indebted to the team of J. Lukes, R. Sobotka and P. Keeling, for giving access to their data concerning the split of the photosystem I reaction center protein prior to publication. Thanks are extended to I. Hunalova and F. Matousek for technical assistance.",

year = "2013",

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doi = "10.1016/j.bbabio.2013.02.002",

language = "English (US)",

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journal = "Biochimica et Biophysica Acta (BBA) - Bioenergetics",

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T1 - Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites

AU - Tichý, Josef

AU - Gardian, Zdenko

AU - Bína, David

AU - Koník, Peter

AU - Litvín, Radek V.

AU - Herbstová, Miroslava

AU - Pain, Arnab

AU - Vácha, František

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): IC/2010/09 Acknowledgements: This research was supported by the GAAV 32-IAA601410907 and GAJU 134/2010/P with institutional support RVO:60077344. Research on C. velia genomics is supported by an Award IC/2010/09 made by the King Abdullah University of Science and Technology (KAUST) to A. Pain. The authors are greatly indebted to the team of J. Lukes, R. Sobotka and P. Keeling, for giving access to their data concerning the split of the photosystem I reaction center protein prior to publication. Thanks are extended to I. Hunalova and F. Matousek for technical assistance.

PY - 2013/6

Y1 - 2013/6

N2 - The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18-19 kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle analysis and circular dichroism spectroscopy indicated similarity of this structure to the recently characterized XLH antenna of xanthophytes. In light of these data we denote this antenna complex CLH, for "Chromera Light Harvesting" complex. The other system was identified as the photosystem I with bound Light Harvesting Complexes (PSI-LHCr) related to the red algae LHCI antennae. The result of this study is the finding that C. velia, when grown in natural light conditions, possesses light harvesting antennae typically found in two different, evolutionary distant, groups of photosynthetic organisms. © 2013 Elsevier B.V. All rights reserved.

AB - The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18-19 kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle analysis and circular dichroism spectroscopy indicated similarity of this structure to the recently characterized XLH antenna of xanthophytes. In light of these data we denote this antenna complex CLH, for "Chromera Light Harvesting" complex. The other system was identified as the photosystem I with bound Light Harvesting Complexes (PSI-LHCr) related to the red algae LHCI antennae. The result of this study is the finding that C. velia, when grown in natural light conditions, possesses light harvesting antennae typically found in two different, evolutionary distant, groups of photosynthetic organisms. © 2013 Elsevier B.V. All rights reserved.

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JO - Biochimica et Biophysica Acta (BBA) - Bioenergetics

JF - Biochimica et Biophysica Acta (BBA) - Bioenergetics

IS - 6

ER -

Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites

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