TY - JOUR
T1 - LHP1 Regulates H3K27me3 Spreading and Shapes the Three-Dimensional Conformation of the Arabidopsis Genome
AU - Veluchamy, Alaguraj
AU - Jégu, Teddy
AU - Ariel, Federico
AU - Latrasse, David
AU - Mariappan, Kiruthiga
AU - Kim, SoonKap
AU - Crespi, Martin
AU - Hirt, Heribert
AU - Bergounioux, Catherine
AU - Raynaud, Cécile
AU - Benhamed, Moussa
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: TJ was Paris-Sud University PhD fellow. The IPS2 benefits from the support of the LabExSaclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).
PY - 2016/7/13
Y1 - 2016/7/13
N2 - Precise expression patterns of genes in time and space are essential for proper development of multicellular organisms. Dynamic chromatin conformation and spatial organization of the genome constitute a major step in this regulation to modulate developmental outputs. Polycomb repressive complexes (PRCs) mediate stable or flexible gene repression in response to internal and environmental cues. In Arabidopsis thaliana, LHP1 co-localizes with H3K27me3 epigenetic marks throughout the genome and interacts with PRC1 and PRC2 members as well as with a long noncoding RNA. Here, we show that LHP1 is responsible for the spreading of H3K27me3 towards the 3’ end of the gene body. We also identified a subset of LHP1-activated genes and demonstrated that LHP1 shapes local chromatin topology in order to control transcriptional co-regulation. Our work reveals a general role of LHP1 from local to higher conformation levels of chromatin configuration to determine its accessibility to define gene expression patterns.
AB - Precise expression patterns of genes in time and space are essential for proper development of multicellular organisms. Dynamic chromatin conformation and spatial organization of the genome constitute a major step in this regulation to modulate developmental outputs. Polycomb repressive complexes (PRCs) mediate stable or flexible gene repression in response to internal and environmental cues. In Arabidopsis thaliana, LHP1 co-localizes with H3K27me3 epigenetic marks throughout the genome and interacts with PRC1 and PRC2 members as well as with a long noncoding RNA. Here, we show that LHP1 is responsible for the spreading of H3K27me3 towards the 3’ end of the gene body. We also identified a subset of LHP1-activated genes and demonstrated that LHP1 shapes local chromatin topology in order to control transcriptional co-regulation. Our work reveals a general role of LHP1 from local to higher conformation levels of chromatin configuration to determine its accessibility to define gene expression patterns.
UR - http://hdl.handle.net/10754/617786
UR - http://dx.plos.org/10.1371/journal.pone.0158936
UR - http://www.scopus.com/inward/record.url?scp=84978743937&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0158936
DO - 10.1371/journal.pone.0158936
M3 - Article
C2 - 27410265
SN - 1932-6203
VL - 11
SP - e0158936
JO - PLoS ONE
JF - PLoS ONE
IS - 7
ER -