Abstract
A novel stationary phase for micro ion chromatography has been prepared by coating a porous poly(butyl methacrylate-co-ethylene dimethacrylate-co-2- acrylamido-2-methyl-1-propanesulfonic acid) monolith with quaternary amine-functionalized latex particles via simple electrostatic binding. This stationary phase enabled the separation of saccharides in the mobile phase with a high-pH value consisting of aqueous ammonia solution in anion-exchange mode using evaporative light scattering for detection. Effects of both porous properties of the monolithic stationary phase and chromatographic conditions on the separation ability were studied. Under optimized conditions, an efficient separation of seven saccharides was achieved in less than 10 min. The stationary phase also enables the separation of saccharides obtained by the enzymatic hydrolysis of corn starch.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 101-106 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 1053 |
| Issue number | 1-2 SPEC. ISS. |
| DOIs | |
| State | Published - Oct 22 2004 |
| Externally published | Yes |
Bibliographical note
Funding Information:Financial support of this work by the Office of Nonproliferation Research and Engineering of the US Department of Energy under Contract No. DE-AC03-76SF00098 and by a grant of the National Institute of General Medical Sciences, National Institutes of Health (GM-48364) is gratefully acknowledged.
Keywords
- Carbohydrates
- Latex
- Monolithic columns
- Stationary phases, LC
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry