Laser-scribed graphene sensor based on gold nanostructures and molecularly imprinted polymers: Application for Her-2 cancer biomarker detection

Abdellatif Ait Lahcen; Sakandar Rauf; Abdulrahman Aljedaibi; José Ilton De Oliveira Filho; Tutku Beduk; Veerappan Mani; Husam N. Alsharee; Khaled N. Salama

doi:10.1016/j.snb.2021.130556

Laser-scribed graphene sensor based on gold nanostructures and molecularly imprinted polymers: Application for Her-2 cancer biomarker detection

Abdellatif Ait Lahcen, Sakandar Rauf, Abdulrahman Aljedaibi, José Ilton De Oliveira Filho, Tutku Beduk, Veerappan Mani, Husam N. Alsharee, Khaled N. Salama

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Laser scribed graphene (LSG) has shown great potential as a sensing platform due to its high sensitivity, simplicity, porosity, and flexibility. In this context, we report a novel biosensing platform that utilizes LSG electrodes modified with nanostructured gold and molecularly imprinted polymer (MIP) to enhance its sensitivity and selectivity. This biomimetic sensing platform is used to detect the human epidermal growth factor receptor 2 (Her-2) protein, a significant breast cancer biomarker. Hence, a simple and accurate biomimetic sensor is developed in this study. To the best of our knowledge, this is the first report on nanostructured gold modified MIP-based LSG sensor for Her-2. LSG electrodes are fabricated by irradiation of a polyimide sheet using a CO2 laser. Nanostructured gold is electrodeposited onto the LSG to enhance its sensitivity and facilitate better Her-2 immobilization on the sensor surface. For MIP preparation, 3, 4-ethylenedioxythiophene (EDOT) was electropolymerized after pre-adsorption of Her-2 on the electrode surface for 20 min. The MIP deposition, removal, and adsorption parameters were investigated and optimized. The developed sensing strategy showed an excellent ability to detect Her-2 in the concentration range from 1 to 200 ng/mL with a LOD of 0.43 ng/mL. The biomimetic sensor showed high selectivity towards the detection of Her-2 in the presence of other interfering molecules and appreciable recovery values of Her-2 in the spiked undiluted human serum samples. Finally, to show the potential application of the developed LSG-AuNS-MIP sensor as a point-of-care device, the sensor is integrated with a homemade open-source electrochemical analyzer KAUSTat to detect Her-2.

Original language	English (US)
Pages (from-to)	130556
Journal	Sensors and Actuators B: Chemical
DOIs	https://doi.org/10.1016/j.snb.2021.130556
State	Published - Aug 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-08-11
Acknowledged KAUST grant number(s): KAUST Sensor Initiative
Acknowledgements: The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative and KAUST visiting student program for supporting this work. We would like to thank Saravanan Yuvaraja for his support for AFM characterization.

ASJC Scopus subject areas

Materials Chemistry
Instrumentation
Surfaces, Coatings and Films
Metals and Alloys
Electronic, Optical and Magnetic Materials
Electrical and Electronic Engineering
Condensed Matter Physics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.snb.2021.130556

https://repository.kaust.edu.sa/bitstream/10754/670543/1/Laser%20scribed_1-s2.0-S0925400521011242-main.pdf

Cite this

@article{389ff6e4dacc496db226d770fc896d83,

title = "Laser-scribed graphene sensor based on gold nanostructures and molecularly imprinted polymers: Application for Her-2 cancer biomarker detection",

abstract = "Laser scribed graphene (LSG) has shown great potential as a sensing platform due to its high sensitivity, simplicity, porosity, and flexibility. In this context, we report a novel biosensing platform that utilizes LSG electrodes modified with nanostructured gold and molecularly imprinted polymer (MIP) to enhance its sensitivity and selectivity. This biomimetic sensing platform is used to detect the human epidermal growth factor receptor 2 (Her-2) protein, a significant breast cancer biomarker. Hence, a simple and accurate biomimetic sensor is developed in this study. To the best of our knowledge, this is the first report on nanostructured gold modified MIP-based LSG sensor for Her-2. LSG electrodes are fabricated by irradiation of a polyimide sheet using a CO2 laser. Nanostructured gold is electrodeposited onto the LSG to enhance its sensitivity and facilitate better Her-2 immobilization on the sensor surface. For MIP preparation, 3, 4-ethylenedioxythiophene (EDOT) was electropolymerized after pre-adsorption of Her-2 on the electrode surface for 20 min. The MIP deposition, removal, and adsorption parameters were investigated and optimized. The developed sensing strategy showed an excellent ability to detect Her-2 in the concentration range from 1 to 200 ng/mL with a LOD of 0.43 ng/mL. The biomimetic sensor showed high selectivity towards the detection of Her-2 in the presence of other interfering molecules and appreciable recovery values of Her-2 in the spiked undiluted human serum samples. Finally, to show the potential application of the developed LSG-AuNS-MIP sensor as a point-of-care device, the sensor is integrated with a homemade open-source electrochemical analyzer KAUSTat to detect Her-2.",

author = "Lahcen, {Abdellatif Ait} and Sakandar Rauf and Abdulrahman Aljedaibi and {De Oliveira Filho}, {Jos{\'e} Ilton} and Tutku Beduk and Veerappan Mani and Alsharee, {Husam N.} and Salama, {Khaled N.}",

note = "KAUST Repository Item: Exported on 2021-08-11 Acknowledged KAUST grant number(s): KAUST Sensor Initiative Acknowledgements: The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative and KAUST visiting student program for supporting this work. We would like to thank Saravanan Yuvaraja for his support for AFM characterization.",

year = "2021",

month = aug,

doi = "10.1016/j.snb.2021.130556",

language = "English (US)",

pages = "130556",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

}

TY - JOUR

T1 - Laser-scribed graphene sensor based on gold nanostructures and molecularly imprinted polymers: Application for Her-2 cancer biomarker detection

AU - Lahcen, Abdellatif Ait

AU - Rauf, Sakandar

AU - Aljedaibi, Abdulrahman

AU - De Oliveira Filho, José Ilton

AU - Beduk, Tutku

AU - Mani, Veerappan

AU - Alsharee, Husam N.

AU - Salama, Khaled N.

N1 - KAUST Repository Item: Exported on 2021-08-11 Acknowledged KAUST grant number(s): KAUST Sensor Initiative Acknowledgements: The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative and KAUST visiting student program for supporting this work. We would like to thank Saravanan Yuvaraja for his support for AFM characterization.

PY - 2021/8

Y1 - 2021/8

N2 - Laser scribed graphene (LSG) has shown great potential as a sensing platform due to its high sensitivity, simplicity, porosity, and flexibility. In this context, we report a novel biosensing platform that utilizes LSG electrodes modified with nanostructured gold and molecularly imprinted polymer (MIP) to enhance its sensitivity and selectivity. This biomimetic sensing platform is used to detect the human epidermal growth factor receptor 2 (Her-2) protein, a significant breast cancer biomarker. Hence, a simple and accurate biomimetic sensor is developed in this study. To the best of our knowledge, this is the first report on nanostructured gold modified MIP-based LSG sensor for Her-2. LSG electrodes are fabricated by irradiation of a polyimide sheet using a CO2 laser. Nanostructured gold is electrodeposited onto the LSG to enhance its sensitivity and facilitate better Her-2 immobilization on the sensor surface. For MIP preparation, 3, 4-ethylenedioxythiophene (EDOT) was electropolymerized after pre-adsorption of Her-2 on the electrode surface for 20 min. The MIP deposition, removal, and adsorption parameters were investigated and optimized. The developed sensing strategy showed an excellent ability to detect Her-2 in the concentration range from 1 to 200 ng/mL with a LOD of 0.43 ng/mL. The biomimetic sensor showed high selectivity towards the detection of Her-2 in the presence of other interfering molecules and appreciable recovery values of Her-2 in the spiked undiluted human serum samples. Finally, to show the potential application of the developed LSG-AuNS-MIP sensor as a point-of-care device, the sensor is integrated with a homemade open-source electrochemical analyzer KAUSTat to detect Her-2.

AB - Laser scribed graphene (LSG) has shown great potential as a sensing platform due to its high sensitivity, simplicity, porosity, and flexibility. In this context, we report a novel biosensing platform that utilizes LSG electrodes modified with nanostructured gold and molecularly imprinted polymer (MIP) to enhance its sensitivity and selectivity. This biomimetic sensing platform is used to detect the human epidermal growth factor receptor 2 (Her-2) protein, a significant breast cancer biomarker. Hence, a simple and accurate biomimetic sensor is developed in this study. To the best of our knowledge, this is the first report on nanostructured gold modified MIP-based LSG sensor for Her-2. LSG electrodes are fabricated by irradiation of a polyimide sheet using a CO2 laser. Nanostructured gold is electrodeposited onto the LSG to enhance its sensitivity and facilitate better Her-2 immobilization on the sensor surface. For MIP preparation, 3, 4-ethylenedioxythiophene (EDOT) was electropolymerized after pre-adsorption of Her-2 on the electrode surface for 20 min. The MIP deposition, removal, and adsorption parameters were investigated and optimized. The developed sensing strategy showed an excellent ability to detect Her-2 in the concentration range from 1 to 200 ng/mL with a LOD of 0.43 ng/mL. The biomimetic sensor showed high selectivity towards the detection of Her-2 in the presence of other interfering molecules and appreciable recovery values of Her-2 in the spiked undiluted human serum samples. Finally, to show the potential application of the developed LSG-AuNS-MIP sensor as a point-of-care device, the sensor is integrated with a homemade open-source electrochemical analyzer KAUSTat to detect Her-2.

UR - http://hdl.handle.net/10754/670543

UR - https://linkinghub.elsevier.com/retrieve/pii/S0925400521011242

U2 - 10.1016/j.snb.2021.130556

DO - 10.1016/j.snb.2021.130556

M3 - Article

SN - 0925-4005

SP - 130556

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Laser-scribed graphene sensor based on gold nanostructures and molecularly imprinted polymers: Application for Her-2 cancer biomarker detection

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this