Laser-Scribed Graphene Electrodes for Aptamer-Based Biosensing

Christoph Fenzl, Pranati Nayak, Thomas Hirsch, Otto S. Wolfbeis, Husam N. Alshareef, Antje J. Baeumner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

113 Scopus citations


Graphene as a transducer material has produced some of the best performing sensing approaches to date opening the door toward integrated miniaturized all-carbon point-of-care devices. Addressing this opportunity, laser-scribed graphene(LSG) electrodes are demonstrated here as highly sensitive and reliable biosensor transducers in blood serum analysis. These flexible electrodes with large electrochemical surface areas were fabricated using a direct-write laser process on polyimide foils. A universal immobilization approach is established by anchoring 1-pyrenebutyric acid to the graphene and subsequently covalently attaching an aptamer against the coagulation factor thrombin as an exemplary bioreceptor to the carboxyl groups. The resulting biosensor displays extremely low detection limits of 1 pM in buffer and 5 pM in the complex matrix of serum.
Original languageEnglish (US)
Pages (from-to)616-620
Number of pages5
JournalACS Sensors
Issue number5
StatePublished - May 26 2017


  • aptamer
  • biosensor
  • carbon nanomaterial
  • electrochemistry
  • immobilization
  • laser-scribed graphene


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