Laser-scribed Graphene Electrodes as an Electrochemical Immunosensing Platform for Cancer Biomarker ‘eIF3d’

Simge Balaban; Tutku Beduk; Ceren Durmus; Eda Aydindogan; Khaled N. Salama; Suna Timur

doi:10.1002/elan.202060482

Laser-scribed Graphene Electrodes as an Electrochemical Immunosensing Platform for Cancer Biomarker ‘eIF3d’

Simge Balaban, Tutku Beduk, Ceren Durmus, Eda Aydindogan, Khaled N. Salama, Suna Timur

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

eIF3d is a protein biomarker which has a potential for the diagnosis of various cancers. Herein, a bio-platform was constructed for eIF3d sensing by using LSG and surface functionalization with anti eIF3d antibody via EDC/NHS chemistry. Following the surface modifications, XPS and several electrochemical methods were used. Difference in the signals were related to biomarker amounts between 75–500 ng/mL. LOD was calculated as 50.4 ng/mL. Selectivity of biosensor was tested by using of various interference molecules. EIF3d was also successfully detected in synthetic biological samples. Thus, to the best of our knowledge, this study is one of the rare studies on use of LSGs in immunosensor studies.

Original language	English (US)
Journal	Electroanalysis
DOIs	https://doi.org/10.1002/elan.202060482
State	Published - Jan 8 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-01-21
Acknowledgements: The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Biosensing experiments as well as SEM and XPS analysis were conducted in Ege University, Central Research Test and Analysis Laboratory Application and Research Center (EGE-MATAL).

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10.1002/elan.202060482

https://repository.kaust.edu.sa/bitstream/10754/666942/1/Laser_Balaban%20et%20al_2020.pdf

Cite this

@article{98b98c2cec2c4dd1813fae7069b613a0,

title = "Laser-scribed Graphene Electrodes as an Electrochemical Immunosensing Platform for Cancer Biomarker {\textquoteleft}eIF3d{\textquoteright}",

abstract = "eIF3d is a protein biomarker which has a potential for the diagnosis of various cancers. Herein, a bio-platform was constructed for eIF3d sensing by using LSG and surface functionalization with anti eIF3d antibody via EDC/NHS chemistry. Following the surface modifications, XPS and several electrochemical methods were used. Difference in the signals were related to biomarker amounts between 75–500 ng/mL. LOD was calculated as 50.4 ng/mL. Selectivity of biosensor was tested by using of various interference molecules. EIF3d was also successfully detected in synthetic biological samples. Thus, to the best of our knowledge, this study is one of the rare studies on use of LSGs in immunosensor studies.",

author = "Simge Balaban and Tutku Beduk and Ceren Durmus and Eda Aydindogan and Salama, {Khaled N.} and Suna Timur",

note = "KAUST Repository Item: Exported on 2021-01-21 Acknowledgements: The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Biosensing experiments as well as SEM and XPS analysis were conducted in Ege University, Central Research Test and Analysis Laboratory Application and Research Center (EGE-MATAL).",

year = "2021",

month = jan,

day = "8",

doi = "10.1002/elan.202060482",

language = "English (US)",

journal = "Electroanalysis",

issn = "1521-4109",

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T1 - Laser-scribed Graphene Electrodes as an Electrochemical Immunosensing Platform for Cancer Biomarker ‘eIF3d’

AU - Balaban, Simge

AU - Beduk, Tutku

AU - Durmus, Ceren

AU - Aydindogan, Eda

AU - Salama, Khaled N.

AU - Timur, Suna

N1 - KAUST Repository Item: Exported on 2021-01-21 Acknowledgements: The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Biosensing experiments as well as SEM and XPS analysis were conducted in Ege University, Central Research Test and Analysis Laboratory Application and Research Center (EGE-MATAL).

PY - 2021/1/8

Y1 - 2021/1/8

N2 - eIF3d is a protein biomarker which has a potential for the diagnosis of various cancers. Herein, a bio-platform was constructed for eIF3d sensing by using LSG and surface functionalization with anti eIF3d antibody via EDC/NHS chemistry. Following the surface modifications, XPS and several electrochemical methods were used. Difference in the signals were related to biomarker amounts between 75–500 ng/mL. LOD was calculated as 50.4 ng/mL. Selectivity of biosensor was tested by using of various interference molecules. EIF3d was also successfully detected in synthetic biological samples. Thus, to the best of our knowledge, this study is one of the rare studies on use of LSGs in immunosensor studies.

AB - eIF3d is a protein biomarker which has a potential for the diagnosis of various cancers. Herein, a bio-platform was constructed for eIF3d sensing by using LSG and surface functionalization with anti eIF3d antibody via EDC/NHS chemistry. Following the surface modifications, XPS and several electrochemical methods were used. Difference in the signals were related to biomarker amounts between 75–500 ng/mL. LOD was calculated as 50.4 ng/mL. Selectivity of biosensor was tested by using of various interference molecules. EIF3d was also successfully detected in synthetic biological samples. Thus, to the best of our knowledge, this study is one of the rare studies on use of LSGs in immunosensor studies.

UR - http://hdl.handle.net/10754/666942

UR - https://onlinelibrary.wiley.com/doi/10.1002/elan.202060482

UR - http://www.scopus.com/inward/record.url?scp=85099235117&partnerID=8YFLogxK

U2 - 10.1002/elan.202060482

DO - 10.1002/elan.202060482

M3 - Article

SN - 1521-4109

JO - Electroanalysis

JF - Electroanalysis

ER -

Laser-scribed Graphene Electrodes as an Electrochemical Immunosensing Platform for Cancer Biomarker ‘eIF3d’

Abstract

Bibliographical note

UN SDGs

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