TY - JOUR
T1 - Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution.
AU - Nelson, David R
AU - Hazzouri, Khaled M
AU - Lauersen, Kyle J
AU - Jaiswal, Ashish
AU - Chaiboonchoe, Amphun
AU - Mystikou, Alexandra
AU - Fu, Weiqi
AU - Daakour, Sarah
AU - Dohai, Bushra
AU - Alzahmi, Amnah
AU - Nobles, David
AU - Hurd, Mark
AU - Sexton, Julie
AU - Preston, Michael J
AU - Blanchette, Joan
AU - Lomas, Michael W
AU - Amiri, Khaled M A
AU - Salehi-Ashtiani, Kourosh
N1 - KAUST Repository Item: Exported on 2021-01-21
Acknowledgements: We thank the NYUAD High-Performance Computing Center for providing computational resources. This work was supported by Tamkeen under the NYU Abu Dhabi Research Institute grant to the NYUAD Center for Genomics and Systems Biology (73 71210 CGSB9), and by NYUAD Faculty Research Funds (AD060).
PY - 2021/1/12
Y1 - 2021/1/12
N2 - Being integral primary producers in diverse ecosystems, microalgal genomes could be mined for ecological insights, but representative genome sequences are lacking for many phyla. We cultured and sequenced 107 microalgae species from 11 different phyla indigenous to varied geographies and climates. This collection was used to resolve genomic differences between saltwater and freshwater microalgae. Freshwater species showed domain-centric ontology enrichment for nuclear and nuclear membrane functions, while saltwater species were enriched in organellar and cellular membrane functions. Further, marine species contained significantly more viral families in their genomes (p = 8e-4). Sequences from Chlorovirus, Coccolithovirus, Pandoravirus, Marseillevirus, Tupanvirus, and other viruses were found integrated into the genomes of algal from marine environments. These viral-origin sequences were found to be expressed and code for a wide variety of functions. Together, this study comprehensively defines the expanse of protein-coding and viral elements in microalgal genomes and posits a unified adaptive strategy for algal halotolerance.
AB - Being integral primary producers in diverse ecosystems, microalgal genomes could be mined for ecological insights, but representative genome sequences are lacking for many phyla. We cultured and sequenced 107 microalgae species from 11 different phyla indigenous to varied geographies and climates. This collection was used to resolve genomic differences between saltwater and freshwater microalgae. Freshwater species showed domain-centric ontology enrichment for nuclear and nuclear membrane functions, while saltwater species were enriched in organellar and cellular membrane functions. Further, marine species contained significantly more viral families in their genomes (p = 8e-4). Sequences from Chlorovirus, Coccolithovirus, Pandoravirus, Marseillevirus, Tupanvirus, and other viruses were found integrated into the genomes of algal from marine environments. These viral-origin sequences were found to be expressed and code for a wide variety of functions. Together, this study comprehensively defines the expanse of protein-coding and viral elements in microalgal genomes and posits a unified adaptive strategy for algal halotolerance.
UR - http://hdl.handle.net/10754/666949
UR - https://linkinghub.elsevier.com/retrieve/pii/S1931312820306673
U2 - 10.1016/j.chom.2020.12.005
DO - 10.1016/j.chom.2020.12.005
M3 - Article
C2 - 33434515
SN - 1931-3128
JO - Cell Host and Microbe
JF - Cell Host and Microbe
ER -
