The Langmuir (L) and Langmuir-Blodgett (LB) properties of a prototypical member of the polyfluorene family of commercially viable organic light emitting diode (OLED) materials, namely poly(9,9-dioctylfluorene) (PFO) are reported. The Langmuir monolayer was characterised by π-A isotherms. UV-Visible absorption spectroscopy demonstrated the existence of PFO within the β-phase conformation (exhibiting extended conjugation lengths) in the Langmuir monolayer on the water surface, as evidenced by the presence of a well-resolved peak at 435 nm. A high boiling point solvent (xylene) was used in the monolayer to facilitate LB film deposition, which was performed on glass and silicon substrates. Langmuir-Blodgett films of PFO were deposited at a range of pressures (2-15 mN m-1) and deposition speeds (100-1000 mm min-1). The LB films were characterised by photoluminescence spectroscopy and decay dynamics, by fluorescence microscopy, and by UV-Visible absorption spectroscopy. The latter measurements demonstrated that the β-phase component remained present after film deposition. © 2003 Elsevier Science B.V. All rights reserved.