Abstract
One important factor for successful disease management is the ability to rapidly and accurately identify the causal agent. Plant viruses cause severe economic losses and pose a serious threat to sustainable agriculture. Therefore, optimization of the speed, sensitivity, feasibility, portability, and accuracy of virus detection is urgently needed. Here, we developed a clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid diagnostic method utilizing the CRISPR–Cas12a system for detecting two geminiviruses, tomato yellow leaf curl virus (TYLCV) and tomato leaf curl New Delhi virus (ToLCNDV), which have single-stranded DNA genomes. Our assay detected TYLCV and ToLCNDV in infected plants with high sensitivity and specificity. Our newly developed assay can be performed in ~1 h and provides easy-to-interpret visual readouts using a simple, low-cost fluorescence visualizer, making it suitable for point-of-use applications.
Original language | English (US) |
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Pages (from-to) | 466 |
Journal | Viruses |
Volume | 13 |
Issue number | 3 |
DOIs | |
State | Published - Mar 12 2021 |
Bibliographical note
KAUST Repository Item: Exported on 2021-03-30Acknowledged KAUST grant number(s): CRG8-URF/4026
Acknowledgements: This research was supported by the King Abdullah University of Science and Technology (KAUST) Competitive Research Grants (CRG8) under Award No. CRG8-URF/4026.
ASJC Scopus subject areas
- Virology
- Infectious Diseases