Abstract
The authors investigated concomitant lactate and glucose metabolism in primary neuronal cultures using 13C-and 1H-NMR spectroscopy. Neurons were incubated in a medium containing either [1- 13C]glucose and different unlabeled lactate concentrations, or unlabeled glucose and different [3-13C]lactate concentrations. Overall, 13C-NMR spectra of cellular extracts showed that more 13C was incorporated into glutamate when lactate was the enriched substrate. Glutamate 13C-enrichment was also found to be much higher in lactate-labeled than in glucose-labeled conditions. When glucose and lactate concentrations were identical (5.5 mmol/L), relative contributions of glucose and lactate to neuronal oxidative metabolism amounted to 21% and 79%, respectively. Results clearly indicate that when neurons are in the presence of both glucose and lactate, they preferentially use lactate as their main oxidative substrate.
Original language | English (US) |
---|---|
Pages (from-to) | 1298-1306 |
Number of pages | 9 |
Journal | Journal of Cerebral Blood Flow and Metabolism |
Volume | 23 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2003 |
Externally published | Yes |
Keywords
- Brain
- Energy metabolism
- Glutamate
- Monocarboxylate
- NMR spectroscopy
- TCA cycle
ASJC Scopus subject areas
- Neurology
- Clinical Neurology
- Cardiology and Cardiovascular Medicine