Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

Etienne Shaffer; Corinne Moratal; Pierre Magistretti; Pierre Marquet; Christian Depeursinge

doi:10.1364/OL.35.004102

Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

Etienne Shaffer^*, Corinne Moratal, Pierre Magistretti, Pierre Marquet, Christian Depeursinge

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.

Original language	English (US)
Pages (from-to)	4102-4104
Number of pages	3
Journal	OPTICS LETTERS
Volume	35
Issue number	24
DOIs	https://doi.org/10.1364/OL.35.004102
State	Published - Dec 15 2010
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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abstract = "We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.",

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AU - Shaffer, Etienne

AU - Moratal, Corinne

AU - Magistretti, Pierre

AU - Marquet, Pierre

AU - Depeursinge, Christian

PY - 2010/12/15

Y1 - 2010/12/15

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AB - We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.

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Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

Abstract

ASJC Scopus subject areas

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